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Merck

06-238

Anti-Gβ Antibody

serum, Upstate®

Synonym(s):

Anti-HG2C1, Anti-NEDHYDF, Anti-SSS4

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
21
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biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

serum

clone

polyclonal

species reactivity

mouse, human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... GNB2(2783)

General description

35/36kDa

Immunogen

Internal sequence of G-protein β subunit (KTREGNVRVSREL) conjugated to octavalent polylysine base

Application

Detect Gβ using this Anti-Gβ Antibody validated for use in IP & WB.

Biochem/physiol Actions

Gβ subunits; recognizes both isoforms

Physical form

PBS, pH 7.2

Analysis Note

Immunoblot Analysis: A 1:500-1:1000 dilution of this antibody detected the Gβ subunits in 20 μg of a cell lysate prepared from rat brain microsomal preparation (Catalog # 12-144).

Immunoprecipitation/Immunoblot: 10 μl of this antibody immunoprecipitated the Gβ subunit from 500 μg-1mg of a lysate of rat brain microsomal preparation (Catalog # 12-144).

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany


Storage Class

10 - Combustible liquids

wgk

WGK 2



Certificates of Analysis (COA)

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M C Galas et al.
The Journal of biological chemistry, 272(5), 2788-2793 (1997-01-31)
The ADP-ribosylation factor (ARF) GTP-binding proteins are believed to function as regulators of vesicular budding and fusion along the secretory pathway. To investigate the role of ARF in regulated exocytosis, we have examined its intracellular distribution in cultured chromaffin cells
H I Krieger-Brauer et al.
The Journal of biological chemistry, 272(15), 10135-10143 (1997-04-11)
Human fat cells possess a multireceptor-linked H2O2-generating system that is activated by insulin. Previous studies revealed that manganese was the sole cofactor required for a hormonal regulation of NADPH-dependent H2O2 generation in vitro. In this report it is shown that
Importance of the G protein gamma subunit in activating G protein-coupled inward rectifier K(+) channels.
T Kawano, L Chen, S Y Watanabe, J Yamauchi, Y Kaziro, Y Nakajima, S Nakajima, H Itoh
Febs Letters null



Global Trade Item Number

SKUGTIN
06-23804053252278464