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Key Documents

06-711

Sigma-Aldrich

Anti-FADD Antibody

Upstate®, from rabbit

Synonym(s):

FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity, Protein FADD

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... FADD(8772)

General description

FAS-associated death domain protein (UniProt: Q13158; also known as FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity, Protein FADD) is encoded by the FADD (also known as MORT1, GIG3) gene (Gene ID: 8772) in human. FADD is an apoptotic adaptor molecule that bridges death receptor signaling to the caspase cascade. It is expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes. FADD contains a death domain at its C-terminus and a death effector domain (DED) at its N-terminal. The death domain is responsible for FADD binding to the activated trimerized Fas receptor. FADD recruits procaspase-8 through its N-terminal DED, which is then activated following the cleavage of pro domain. The death inducing signaling complex (DISC) formed by Fas receptor, FADD, and caspase-8 is essential in triggering apoptotic events in cells. Human FADD is phosphorylated mainly at Ser194. Defects in FADD gene have been linked to recurrent infections, hepatic dysfunction, and cardiovascular malformations. These subjects display high circulating levels of CD4(-) and CD8(-) cells and elevated levels of IL-10.
~26 kDa observed; 23.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Specificity

This polyclonal antibody detects FADD protein in human cells.

Immunogen

His-tagged full-length human recombinant FAS-associated death domain protein.

Application

Detect FAS-associated death domain protein using this rabbit polyclonal Anti-FADD, Cat. No. 06-711. It has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected FADD in human pancreas, human kidney, and human prostate tissue.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Linkage

Replaces: 04-1113

Physical form

ImmunoAffinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected FADD in 10 µg of Jurkat cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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