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Merck

06-872

Anti-IRAK Antibody

Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Anti-IRAK Antibody, Upstate®, from rabbit

biological source

rabbit

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_001569

UniProt accession no.

P51617

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

mouse ... Clec1B(56760)

Analysis Note

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
routinely evaluated by immunoblot on HeLa nuclear extract

Application

Detect IRAK using this Anti-IRAK Antibody validated for use in IP & WB.
Research Category
Signaling
Research Sub Category
Immunological Signaling

Biochem/physiol Actions

Recognizes IRAK.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

80 kDa

Immunogen

Peptide (DRQGPEESDEFQS) corresponding to amino acids 700-712 of human IRAK

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Ammonium sulfate precipitation and DEAE-cellulose chromatography
Format: Purified
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Preparation Note

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Candida albicans triggers activation of distinct signaling pathways to establish a proinflammatory gene expression program in primary human endothelial cells.
Verena Muller, Dorothee Viemann, Marc Schmidt, Nicole Endres, Stephan Ludwig et al.
Journal of immunology (Baltimore, Md. : 1950) (1950)
M Trofimova et al.
The Journal of biological chemistry, 271(30), 17609-17612 (1996-07-26)
The NF-kappaB/c-Rel proteins are a family of evolutionarily conserved transcription factors activated during development that in the adult, mediate many processes including the immune response. A high degree of sequence similarity is shared between the NF-kappaB/c-Rel family of transcription factors
Bruns A Watts et al.
American journal of physiology. Renal physiology, 317(3), F705-F719 (2019-06-27)
LPS inhibits HCO3- absorption in the medullary thick ascending limb (MTAL) through a Toll-like receptor 4 (TLR4)-myeloid differentiation factor 88 (MyD88)-extracellular signal-regulated kinase (ERK) pathway that is upregulated by sepsis. Pretreatment with the nontoxic immunomodulator monophosphoryl lipid A (MPLA) prevents
Neutrophil signaling pathways activated by bacterial DNA stimulation.
Maria E Alvarez, Juan I Fuxman Bass, Jorge R Geffner, Paula X Fernandez Calotti et al.
Journal of immunology (Baltimore, Md. : 1950) (1950)
L Li et al.
The Journal of biological chemistry, 275(30), 23340-23345 (2000-05-16)
Interleukin-1 receptor-associated kinase (IRAK), a signal transducer for interleukin-1, has also been suggested to participate in the Toll-like receptor-mediated innate immune response to bacterial endotoxin lipopolysaccharide (LPS). Using the human promonocytic THP-1 cell line, we demonstrated that the endogenous IRAK
View All Related Papers

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Pathways and Biomarkers of Toll-like Receptor (TLR) Signaling

"Toll-like Receptors (TLRs) are transmembrane proteins that are expressed on various immune cells. The extracellular N-terminal region of TLRs recognizes specific pathogen components. At least 13 different members of TLR family have been identified that detect different pathogen associated molecular patterns (PAMPs), including lipopolysaccharides, flagellin, bacterial CpG DNA, and viral RNA and DNA. Recognition of PAMPs by TLRs is considered as a key process for the induction of an inflammatory response."

Global Trade Item Number

SKUGTIN
06-87204053252674334

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