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Merck

07-1014

Anti-Upf1 Antibody

from rabbit

Synonym(s):

ATP-dependent helicase RENT1, Nonsense mRNA reducing factor 1, UP Frameshift 1, UPF1 regulator of nonsense transcripts homolog (yeast), Up-frameshift suppressor 1 homolog, delta helicase, regulator of nonsense transcripts 1, up-frameshift mutation 1 homo

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

species reactivity (predicted by homology)

mouse, bovine, canine, rat

technique(s)

western blot: suitable

NCBI accession no.

NP_002902.2

UniProt accession no.

Q92900

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... UPF1(5976)

General description

Upf1 is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. Upf1 is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping.
~125 kDa

Immunogen

KLH-conjugated synthetic linear peptide corresponding to Upf1.

Application

Anti-Upf1 Antibody is a rabbit polyclonal antibody for detection of Upf1 also known as ATP-dependent helicase RENT1 ,Nonsense mRNA reducing factor 1, Up-frameshift suppressor 1 homolog & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Binding Protein (RBP)

Biochem/physiol Actions

This antibody detects Upf1.

Physical form

Affinity purified
Format: Purified
Purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
U2OS cell lysate
Evaluated by western blot on U2OS cell lysate.

Western Blot Analysis: 0.5-2 µg/mL of this antibody detected Upf1 in U2OS cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Gabriel Sanchez et al.
Nucleic acids research, 44(6), 2661-2676 (2015-12-15)
Loss of 'Survival of Motor Neurons' (SMN) leads to spinal muscular atrophy (SMA), a disease characterized by degeneration of spinal cord alpha motor neurons, resulting in muscle weakness, paralysis and death during early childhood. SMN is required for assembly of
Catia Andreassi et al.
Cell reports, 34(8), 108778-108778 (2021-02-25)
The 3' untranslated regions (3' UTRs) of messenger RNAs (mRNAs) are non-coding sequences involved in many aspects of mRNA metabolism, including intracellular localization and translation. Incorrect processing and delivery of mRNA cause severe developmental defects and have been implicated in
Bonnie R Fletcher et al.
Neurobiology of learning and memory, 115, 58-67 (2014-08-26)
Memory decline is a common feature of aging. Expression of the immediate-early gene Arc is necessary for normal long-term memory, and although experience dependent Arc transcription is reportedly reduced in the aged rat hippocampus, it has not been clear whether

Global Trade Item Number

SKUGTIN
07-101404053252512971

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