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Merck

07-108

Anti-Histone H4 Antibody

Upstate®, from rabbit

Synonym(s):

H4 histone family, member A, histone 1, H4a, histone cluster 1, H4a

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Anti-Histone H4 Antibody, Upstate®, from rabbit

biological source

rabbit

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

Xenopus, mouse, avian, chicken, human, bovine

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NP_778224

UniProt accession no.

P62805

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

bovine ... H4C1(617905)
frog ... H4C1(549623)
human ... H4C1(8359)
mouse ... H4C1(326619)

Analysis Note

Control
HeLa cell extract.
Routinely evaluated by western blot on acid extracts of HeLa cells treated with sodium butyrate.

Western Blot Analysis:
0.1-0.5 μg/mL of this lot detected Histone H4 in acid extracts of HeLa cells treated with or without 5 mM sodium butyrate. This lot did not cross react with Histone H2A, Histone H2B or Histone H3.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-Histone H4 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect Histone H4 also known as Histone H4.

Biochem/physiol Actions

Broad species cross-reactivity expected.
Recognizes Histone H4, Mr 10 kDa.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
10 kDa

Immunogen

Calf thymus histone H4.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Protein A chromatography
Purified rabbit polyclonal IgG in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% Sodium Azide.

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Regulation of chemokine gene expression by hypoxia via cooperative activation of NF-kappaB and histone deacetylase.
Olga Safronova,Sireerat Pluemsampant,Ken-ichi Nakahama,Ikuo Morita
The International Journal of Biochemistry & Cell Biology null
S L Gilbert et al.
The Journal of biological chemistry, 275(47), 36491-36494 (2000-09-28)
Microscopy studies have shown that XIST RNA colocalizes with the inactive X chromosome (Xi). However, the molecular basis for this colocalization is unknown. Here we provide two lines of evidence from chromatin immunoprecipitation experiments that XIST RNA physically associates with
Ingeborg M M van Leeuwen et al.
Molecular cancer therapeutics, 12(4), 471-480 (2013-02-19)
Acetylation of C-terminal lysine residues in the p53 tumor suppressor is associated with increased stability and transcription factor activity. The function, protein level, and acetylation of p53 are downregulated by mdm2, which in its turn is inhibited by the p14(ARF)
Histone deacetylases and ASYMMETRIC LEAVES2 are involved in the establishment of polarity in leaves of Arabidopsis.
Ueno, Y; Ishikawa, T; Watanabe, K; Terakura, S; Iwakawa, H; Okada, K; Machida, C; Machida, Y
Plant Cell null
Setdb1-mediated histone H3K9 hypermethylation in neurons worsens the neurological phenotype of Mecp2-deficient mice.
Jiang, Y; Matevossian, A; Guo, Y; Akbarian, S
Neuropharmacology null
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Global Trade Item Number

SKUGTIN
07-10804053252678653

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