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Merck

07-1378

Anti-QIK (SIK2) Antibody

Upstate®, from rabbit

Synonym(s):

Qin-induced kinase, SNF1-like kinase 2, Salt-inducible protein kinase 2, salt-inducible kinase 2, salt-inducible serine/threonine kinase 2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, canine, mouse, chicken, bovine, human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

NCBI accession no.

NP_056006

UniProt accession no.

Q9H0K1

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SIK2(23235)

General description

104 kDa
QIK (Qin-induced Kinase, SNF1LK2, SIK2, Salt-induced protein Kinase 2) is a Ser/Thr kinase that belongs to the AMPK/SNF1 kinase family. QIK is phosphorylated and activated by the tumor suppressor LKB1 when in complex with the pseudokinase STRAD and the scaffolding protein MO25. QIK has been found in adipocytes where it is known to phosphorylate a few substrates that include insulin receptor substrate 1 (IRS1) on Ser794 and TORC2 (also known as CRTC2 and not to be confused with the mTOR complex) on Ser171. The phosphorylation of TORC2 results in its translocation to the nucleus where it binds to CREB.

Immunogen

KLH-conjugated synthetic peptide corresponding to the C-term of human QIK.

Application

Anti-QIK (SIK2) Antibody is an antibody against QIK (SIK2) for use in WB.
Research Category
Signaling

Biochem/physiol Actions

Close homology with mouse, rat, chicken, dog, and cow.
Detects QIK

Physical form

Format: Purified
Protein A purified
Protein A purified rabbit serum in 0.1M Tris-Glycine (pH7.4) 15mM NaCl, and 0.05% NaN3 with 30% glycerol.

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa Cell Lysate
Western Blot Analysis:
This lot detected QIK at 1:1,000-1:2,000 dilution in HeLa lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Salt-inducible kinase in steroidogenesis and adipogenesis.
Okamoto, Mitsuhiro, et al.
Trends in Endocrinology and Metabolism, 15, 21-26 (2004)
Minjeong Park et al.
Scientific reports, 10(1), 8676-8676 (2020-05-28)
Although sleep is one of the most conserved behaviors, the intracellular mechanism regulating sleep/wakefulness remains unknown. We recently identified a protein kinase, SIK3, as a sleep-regulating molecule. Mice that lack a well-conserved protein kinase A (PKA) phosphorylation site, S551, showed

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