07-1385

Anti-Lin28 Antibody

Name: Anti-Lin28 Antibody
Brand: MM

serum, from rabbit

Synonym(s):

RNA-binding protein LIN-28

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_078950

UniProt accession no.

Q9H9Z2

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... LIN28A(79727)

General description

25 kDa

Lin28 is a developmentally regulated RNA binding protein (RBP). Its down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells (Viswanathan, 2008). Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers (Viswanathan, 2008). The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance (Wu, 2005). Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells.

Immunogen

Full-length His-tagged Lin28 recombinant protein (human).

Application

Anti-Lin28 Antibody detects level of Lin28 & has been published & validated for use in WB & IP.

Immunoprecipitation:
Independent lab validated this antibody in immunoprecipitation. The antibody immunoprecipitated Lin28 from Tera-2 embryonic carcinoma and mouse ES cells using 2μL of antibody per reaction.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Signaling Neuroscience

RNA Metabolism & Binding Proteins

RNA Binding Protein (RBP)

Biochem/physiol Actions

Detects Lin28

Other species have not been tested.

Physical form

Rabbit Serum

Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt. Store at -80°C for longer term storage. Avoid repeated freeze/thaws.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Analysis Note

Western Blot Analysis:
This lot detected Lin28 at 1:1,000 dilution in Tera-2 embryonic carcinoma cell lysate resolved via SDS-PAGE and transferred to PVDF.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

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ARiBo pull-down for riboproteomic studies based on label-free quantitative mass spectrometry.

Geneviève Di Tomasso et al.

RNA (New York, N.Y.), 22(11), 1760-1770 (2016-09-24)

As part of their normal life cycle, most RNA molecules associate with several proteins that direct their fate and regulate their function. Here, we describe a novel method for identifying proteins that associate with a target RNA. The procedure is

Selective blockade of microRNA processing by Lin28.

Srinivas R Viswanathan et al.

Science (New York, N.Y.), 320(5872), 97-100 (2008-02-23)

MicroRNAs (miRNAs) play critical roles in development, and dysregulation of miRNA expression has been observed in human malignancies. Recent evidence suggests that the processing of several primary miRNA transcripts (pri-miRNAs) is blocked posttranscriptionally in embryonic stem cells, embryonal carcinoma cells

Depletion of human micro-RNA miR-125b reveals that it is critical for the proliferation of differentiated cells but not for the down-regulation of putative targets during differentiation.

Yong Sun Lee et al.

The Journal of biological chemistry, 280(17), 16635-16641 (2005-02-22)

Micro-RNAs are small non-coding RNAs that regulate target gene expression post-transcriptionally through base pairing with the target messenger RNA. Functional characterization of micro-RNAs awaits robust experimental methods to knock-down a micro-RNA as well as to assay its function in vivo.

Micro-RNA regulation of the mammalian lin-28 gene during neuronal differentiation of embryonal carcinoma cells.

Ligang Wu et al.

Molecular and cellular biology, 25(21), 9198-9208 (2005-10-18)

Vertebrate genomes each encode hundreds of micro-RNAs (miRNAs), yet for few of these miRNAs is there empirical evidence as to which mRNA(s) they regulate. Here we report the identification of human lin-28 mRNA as a regulatory target of human miR-125b

Global Trade Item Number

SKU	GTIN
07-1385	04053252281648

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