Skip to Content
Merck

07-1387

Anti-oxidized-CaM Kinase II (Met281/282) Antibody

serum, from rabbit

Synonym(s):

CAMKK beta protein, CaM-KK beta, CaM-kinase kinase beta, CaMKK beta, Calcium/calmodulin-dependent protein kinase kinase beta, calcium/calmodulin-dependent protein kinase beta, calcium/calmodulin-dependent protein kinase kinase 2 beta, calcium/calmodulin-

Sign Into View Organizational & Contract Pricing

Select a Size

All Photos(2)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat, human

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

NP_742127

UniProt accession no.

Q96RR4

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CAMK2D(817)

General description

The Ca2+/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Ca2+/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions.
60 kDa

Immunogen

Epitope: Oxidized Met281/282
Synthetic peptide encompassing and including oxidized Met281/282.

Application

An independent lab validated this antibody to work in immunocytochemistry in heart sections from mice treated with saline, AngII,or Iso (Erickson, J.R., 2008).
This Anti-oxidized-CaM Kinase II (Met281/282) Antibody is validated for use in WB, IC for the detection of oxidized-CaM Kinase II (Met281/282).

Biochem/physiol Actions

Expected to cross-react on Mouse and Rat based on 100% homology with immunogen sequence.
This antibody recognizes CaM Kinase II when oxidized on Met281/282.

Preparation Note

Stable for 1 years at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Analysis Note

Evaluated by western blot on untreated and H2O2-treated mouse heart cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect oxidized CaM Kinase II in mouse heart cell lysates.

Other Notes

Replaces: 04-1079

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Shuo Geng et al.
Science advances, 5(2), eaav2309-eaav2309 (2019-02-19)
Nonresolving inflammation perpetuated by innate leukocytes is involved in the pathogenesis of unstable atherosclerosis. However, the role and regulation of neutrophils related to nonresolving inflammation and atherosclerosis are poorly understood. We report herein that chronic subclinical endotoxemia, a risk factor
Ichiro Matsuo et al.
PloS one, 17(6), e0258823-e0258823 (2022-06-02)
Oral infections, particularly periodontitis, are a well-established risk factor for cardiovascular diseases, although the molecular mechanisms involved remain elusive. The aims of the present study were to investigate the effects of lipopolysaccharide derived from Porphyromonas gingivalis (PG-LPS) on cardiac function
Xueping Liu et al.
Heliyon, 10(1), e23634-e23634 (2024-01-08)
Airway epithelial cells play important roles in allergic asthma. Transient receptor potential melastatin-related 2 (TRPM2) and oxidized Ca2+/calmodulin-dependent protein kinase Ⅱ (ox-CaMKⅡ) participate in the airway inflammation. This study aimed to analyze the effects of TRPM2 on ox-CaMKⅡ in the
Yao Wu et al.
BioMed research international, 2017, 4150158-4150158 (2017-05-26)
Background. This study was undertaken to determine relative contributions of phosphorylation and oxidation to the increased activity of calcium/calmodulin-stimulated protein kinase II (CaMKII) in juveniles with cardiac myocyte dysfunction due to increased pressure overload. Methods. Juvenile rats underwent abdominal aortic
Gabrielle Fredman et al.
Nature communications, 7, 12859-12859 (2016-09-24)
Chronic unresolved inflammation plays a causal role in the development of advanced atherosclerosis, but the mechanisms that prevent resolution in atherosclerosis remain unclear. Here, we use targeted mass spectrometry to identify specialized pro-resolving lipid mediators (SPM) in histologically-defined stable and
View All Related Papers

Global Trade Item Number

SKUGTIN
07-138704053252403118

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service