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Key Documents

07-1458

Sigma-Aldrich

Anti-ROCK-1/2 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Renal carcinoma antigen NY-REN-35, Rho kinase, Rho-associated, coiled-coil containing protein kinase 1, Rho-associated, coiled-coil-containing protein kinase 1, p160 ROCK-1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

technique(s)

western blot: suitable

NCBI accession no.

NP_005397.1

UniProt accession no.

Q13464

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ROCK1(6093)
mouse ... Rock1(19877)

General description

158 kDa
Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin. The serine-threonine kinase ROCK-1 is a down-stream effector of Rho, and is involved in signal transduction pathways that regulate cell morphology, motility, and tumor progression through the reorganization of the actin cytoskeleton.

Immunogen

Synthetic peptide corresponding to human ROCK-1, 90.5% homology with ROCK-2

Application

This Anti-ROCK-1/2 Antibody is validated for use in WB for the detection of ROCK-1/2.

Biochem/physiol Actions

This antibody recognizes ROCK-1 and ROCK-2.

Analysis Note

Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: 1:500 dilution of this antibody detected ROCK-1/2 on 10 µg of A431 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1121

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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