07-1473
Anti-Phosphothreonine-Proline Antibody
from rabbit, purified by affinity chromatography
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
mouse, rat, human
Application:
WB
Citations:
-
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat, human
technique(s)
western blot: suitable
shipped in
wet ice
target post-translational modification
phosphorylation (pThr)
Quality Level
General description
Phosphothreonine-Proline, along with similar motifs involving serine/threonine residues preceding proline undergoing phosphorylation, has critical roles in the moderation of various cellular processes, protein function, and kinases through proline-directed phosphorylation. The deregulation of proline-directed phosphorylation is known to be a factor in diseases such as cancer and Alzheimer’s neurodegeneration.
Immunogen
KLH-phosphothreonine-proline amide
(pT-P-NH2) peptide conjugates
(pT-P-NH2) peptide conjugates
Application
Detect Phosphothreonine-Proline using this Anti-Phosphothreonine-Proline Antibody validated for use in WB.
Biochem/physiol Actions
Recognizes the phosphorylated phothreonine-proline motif, which is the site of phosphorylation of many proline-directed protein kinase such MAPK and cyclin-dependent protein kinases. Also recognizes the phosphoserine-proline motif to a similar degree.
Physical form
Liquid in TBS with 0.05% sodium azide and 50% glycerol.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Evaluated by Western Blot in Calyculin treated Jurkat cell lysate.
Western Blot Analysis: 1:500 dilution of this lot detected phosphorylated proteins on 10 μg of calyculin treated Jurkat lysates.
Western Blot Analysis: 1:500 dilution of this lot detected phosphorylated proteins on 10 μg of calyculin treated Jurkat lysates.
Control
Calyculin treated Jurkat cell lysate
Calyculin treated Jurkat cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB3192
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 07-1473 | 04053252584855 |
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