Anti-phospho Histone H2A.X Antibody (Tyr142)

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The phosphorylated form of H2A.X is thought to be invloved with DNA repair, possibly through reorganizing chromatin into a more open state and allowing the recruitment of proteins to repair DNA double stranded breaks.

~16 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

KLH-conjugated linear peptide corresponding to the C-terminus of human phospho Histone H2A.X phosphorylated at Tyr142.

Anti-phospho Histone H2A.X Antibody (Tyr142) is a highly specific rabbit polyclonal antibody, that targets Histone H2A.X & has been tested in western blotting & Dot Blot.

Dot Blot Analysis: 0.5 µg/mL from a representative lot detected phospho Histone H2A.X (Tyr142) peptides, but not phospho Histone H2A.X (Tyr142) peptides or other peptides corresponding to modified histones.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in colcemid treated HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected phospho Histone H2A.X (Tyr142) in colcemid treated HeLa cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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