07-225
Anti-TrkB Antibody
Upstate®, from rabbit
Synonym(s):
BDNF/NT-3 growth factors receptor, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, neurotrophic tyrosine kinase receptor, type 2, tyrosine kinase receptor B
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... NTRK2(4915)
General description
145 κDa
The Trk (tropomyosin-receptor-kinase) proto-oncogene family of receptor tyrosine kinases contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkB is the high-affinity to the binding brain-derived neurotrophic factor (BDNF) receptor and neurotrophin-4/5 NT-4/5). TrkB is autophosphorylated on five cytoplasmic tyrosines. The binding of BDNF to TrkB receptor causes many intercellular cascades to be activated such as promoting survival of sensory neurons mainly through Shc site-independent pathways.The signaling activity of these receptors includes the Ras – MAP kinase pathway, and the PI3 Kinase pathway. These receptors are responsible for resistance to apoptosis, obesity, Alzheimer’s disease, and mood disorders.
Immunogen
The entire extracellular domain (corresponding to residues 1–429) of the rat TrkB receptor, expressed in COS cells. The immunizing sequence has 97% identity with mouse TrkB and 88% identity with human TrkB.
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neurochemistry & Neurotrophins
This Anti-TrkB Antibody is validated for use in WB for the detection of TrkB.
Biochem/physiol Actions
Recognizes TrkB, Mr 145 kDa, as well as an additional protein, Mr 90 kDa, which is likely a TrkB degradation product.
Physical form
Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol.
Preparation Note
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
Brain tissue.
Brain tissue.
Evaluated by western blot on mouse brain membrane protein preparations.
Western Blot Analysis:
0.5-2 µg/mL of this antibody detected TrkB in mouse brain membrane protein preparations.
Western Blot Analysis:
0.5-2 µg/mL of this antibody detected TrkB in mouse brain membrane protein preparations.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Conventional kinesin-I motors participate in the anterograde axonal transport of neurotrophins in the visual system.
Rafal Butowt, Christopher S von Bartheld
Journal of Neuroscience Research null
J R Huie et al.
Neuroscience, 200, 74-90 (2011-11-08)
Brain-derived neurotrophic factor (BDNF) has been characterized as a potent modulator of neural plasticity in both the brain and spinal cord. The present experiments use an in vivo model system to demonstrate that training with controllable stimulation increases spinal BDNF
Evidence that biological activity of NGF is mediated through a novel subclass of high affinity receptors
Weskamp, G and Reichardt, L F
Neuron, 6, 649-663 (1991)
JIP3 mediates TrkB axonal anterograde transport and enhances BDNF signaling by directly bridging TrkB with kinesin-1.
Huang, SH; Duan, S; Sun, T; Wang, J; Zhao, L; Geng, Z; Yan, J; Sun, HJ; Chen, ZY
The Journal of Neuroscience null
M Cazorla et al.
British journal of pharmacology, 162(4), 947-960 (2010-11-03)
By interacting with trkB receptors, brain-derived neurotrophic factor (BDNF) triggers various signalling pathways responsible for neurone survival, differentiation and modulation of synaptic transmission. Numerous reports have implicated BDNF and trkB in the pathogenesis of various central nervous system affections and
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