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07-2271

Sigma-Aldrich

Anti-Serine/threonine-protein kinase 39 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

serine threonine kinase 39, proline-alanine-rich STE20-related kinase, Serine/threonine-protein kinase 39, serine threonine kinase 39 (STE20/SPS1 homolog, yeast), Ste-20-related kinase, Ste20-like protein kinase, small intestine SPAK-like kinase, STE20/S

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702

biological source

rabbit

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

western blot: suitable

NCBI accession no.

NP_037365

UniProt accession no.

Q9UEW8

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... STK39(27347)

General description

STE20/SPS-1 related proline alanine-rich kinase (SPAK), or commonly known as Serine/threonine-protein kinase 39, is a member of the SPS1 subfamily of STE20 kinases. Serine/threonine-protein kinase 39 is also very highly conserved between species and has been shown to activate the p38 pathways. Serine/threonine-protein kinase 39 also has a caspase cleavage site and these two functions may mean that Serine/threonine-protein kinase 39 plays a role in stress-activated signaling. Serine/threonine-protein kinase 39 expression is up-regulated by androgens in LNCaP human prostate cancer cells and regulated and phosphorylated by WNK1, which controls the stress signaling pathway. It is activated by WNK4 and then has the ability to stimulate the Na-K-2Cl cotransporter, the inactive version does not posses this ability. Recent research has also made a connection between Serine/threonine-protein kinase 39 and regulation of blood pressure.
~60 kDa observed

Immunogen

GST-tagged recombinant protein corresponding to human Serine/threonine-protein kinase 39.

Application

This Serine/threonine-protein kinase 39 antibody is validated for use in WB for the detection of the Serine/threonine-protein kinase 39 protein.

Biochem/physiol Actions

Other homologies: Mouse (95% sequence homology). Rat (94% sequence homology).

Analysis Note

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 1:900 dilution of this antibody detected Serine/threonine-protein kinase 39 on 10 µg of HEK293 cell lysate.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Eva Dizin et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(2), 2408-2424 (2020-01-08)
The mechanism of sodium retention and its location in kidney tubules may vary with time in nephrotic syndrome (NS). We studied the mechanisms of sodium retention in transgenic POD-ATTAC mice, which display an inducible podocyte-specific apoptosis. At day 2 after

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