07-357
Anti-phospho-eNOS/NOS III (Ser114) Antibody
Upstate®, from rabbit
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human, bovine
Application:
WB
Citations:
3
biological source
rabbit
Quality Segment
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, bovine
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pSer114)
Gene Information
bovine ... Nos3(287024)
human ... NOS3(4846)
General description
132 kDa
Note: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS
Immunogen
Synthetic peptide corresponding to amino acids 106-118 of human eNOS/NOS III (Ser114) (PRKLQGRPpSPGPP-C) with a C-terminal cysteine added for conjugation to KLH. The immunizing sequence shares 12/13 amino acids with pig and bovine eNOS/NOS III.
Application
Detect phospho-eNOS/NOS III (Ser114) using this Anti-phospho-eNOS/NOS III (Ser114) Antibody validated for use in WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Oxidative Stress
Oxidative Stress
Biochem/physiol Actions
eNOS/NOS III phosphorylated on serine 114
Physical form
0.2 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.1 mM EDTA, and 0.05% sodium azide before the addition of glycerol to 30%
Immunoaffinity Chromatography
Preparation Note
1 year at -20°C
Analysis Note
routinely evaluated by immunoblot in fetal bovine heart endothelial cell lysate
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
Certificates of Analysis (COA)
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