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Merck

07-468

Anti-HuR Antibody

Upstate®, from rabbit

Synonym(s):

ELAV-like protein 1, Hu-antigen R

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-HuR Antibody, Upstate®, from rabbit

biological source

rabbit

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_001410

UniProt accession no.

Q15717

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... ELAVL1(1994)

Analysis Note

Western Blot Analysis: Evaluated by Western Blot in 3T3/A31 cell lysate using 1 µg/mL of antibody and 10 µg of lysate.
Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Application

Immunoprecipitation Analysis:
10 µg from a representative lot detected HuR in RIPA lysate.

Immunocytochemistry Analysis:
A 1:500 dilution from a representative lot detected HuR in HeLa and NIH/3T3 cell lysates.

Immunohistochemistry Analysis:
This antibody has been reported by an independent laboratory to detect HuR in rabbit placental tissue.
Anti-HuR Antibody is a Rabbit Polyclonal Antibody for detection of HuR also known as ELAV-like protein 1, Hu-antigen R & has been validated in IP, WB, ICC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

RNA Binding Protein (RBP)

Biochem/physiol Actions

HuR

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

HuR (Hu-antigen R, ELAV-like protein 1, ELAV1) is an RNA binding protein that contains 3 RNA-binding domains. It is known to binds AU-rich elements if mRNA. It regulates gene expression by destabilizing their mRNA. It is known to be upregulated in certain cancers such as breast and gastric cancers. Some of its targets include cFos, IL-3, and BRCA1 mRNAs. HuR has also been shown to be involved in the regulation of VEFG-A and NFkB expression in certain cell types. It activation and accumulation are also linked to many energy signaling molcules such as that of the PI3K/Akt pathway and AMPK..
~36 kDa

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 1-13 of human HuR.

Physical form

Format: Purified
See datasheet for exact formulation.
See datasheet for specific purification method.

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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RNA-Binding Protein Immunoprecipitation

All eukaryotic organisms require tight regulation of gene expression for complex processes such as development, differentiation, cell specification, and responses to environmental stimuli. Many genes are regulated post-transcriptionally, in addition to transcriptional mechanisms of gene regulation. RNA-binding proteins (RBPs) are essential for post-transcriptional gene regulation, linking transcription and translation in many processes including transcription, splicing, export, rate of translation and turnover. In all of these events, RBPs coordinate regulation of the amount of protein produced from mRNA transcripts.

Global Trade Item Number

SKUGTIN
07-46804053252373077

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