07-502
Anti-p67-phox (mouse) Antibody
Upstate®, from rabbit
Synonym(s):
67 kDa neutrophil oxidase factor, NADPH oxidase activator 2, Neutrophil NADPH oxidase factor 2, chronic granulomatous disease, autosomal 2, neutrophil cytosol factor 2, neutrophil cytosolic factor 2, neutrophil cytosolic factor 2 (65kD, chronic granuloma
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Ncf2(17970)
rat ... Ncf2(364018)
General description
67 kDa
Activation of NADPH-oxidase dependent superoxide generation requires the participation of several cytosolic factors including p47phox, p67phox, p40phox and Rac (1 or 2). In the resting cell, p47phox, p67phox, and p40phox form a large molecular weight complex which can be recovered from the cytosolic fraction. Activation of the NADPH oxidase is initiated by the assembly of p47phox, p67phox, and Rac with cytochrome b558 in a 1:1:1:1 complex in the plasma membrane. p47phox, p67phox, and Rac exhibit cooperative binding, since increasing the concentration of any one component lowers the EC50 of the other components. A great deal of current research involves understanding the protein protein interactions, and how these change with the activation state.
Immunogen
KLH-conjugated, synthetic peptides corresponding to the first 15 amino acids from the N-terminal and the last 15 amino acids on the C-terminal of mouse p67-phox.
Application
Detect p67-phox (mouse) using this Anti-p67-phox (mouse) Antibody validated for use in WB, IH.
Immunohistochemistry: This antibody has been demonstrated by an independent laboratory to detect p67-phox in male rat brain cortex tissue.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Oxidative Stress
Oxidative Stress
Biochem/physiol Actions
Recognizes p67-phox, MW ~67 kDa.
Physical form
Format: Purified
Protein A purified
Purified rabbit polyclonal IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat brain tissue, RAW 264.7 cell lysate.
Rat brain tissue, RAW 264.7 cell lysate.
Evaluated by western blot in RIPA lysates from RAW 264.7 cells.
Western Blot Analysis: 0.5-2.0 µg/mL of this antibody detected p67-phox in RIPA lysates from RAW 264.7 cells.
Western Blot Analysis: 0.5-2.0 µg/mL of this antibody detected p67-phox in RIPA lysates from RAW 264.7 cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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The superoxide-generating NADPH oxidase: structural aspects and activation mechanism.
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