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Merck

07-590

Anti-Ago 2 Antibody

Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NP_036286.2

UniProt accession no.

Q9UKV8

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... AGO2(27161)

General description

100 kDa
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.

Provides endonuclease activity to RNA-induced silencing complexes (RISC). Cleaves siRNA/mRNA heteroduplexes bound to RISC. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).

Immunogen

GST fusion protein corresponding to residues 7-48 of human argonaute 2 (Ago2).

Application

Anti-Ago 2 Antibody is a Rabbit Polyclonal Antibody for detection of Ago 2 also known as argonaute 2 & has been tested in WB.

Biochem/physiol Actions

Recognizes Ago 2, also known as eukaryotic translation initiation factor 2C, 2 (EIF2C 2), MGC3183 and Q10.

Physical form

Format: Purified

Analysis Note

Control
HeLa cell lysate, HL-60 cell lysate
Routinely evaluated by immunoblot in RIPA and cytosolic lysates from HeLa cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kumi Sakurai et al.
Nucleic acids research, 39(4), 1510-1525 (2010-10-26)
RNA interference is a powerful mechanism for sequence-specific inhibition of gene expression. It is widely known that small interfering RNAs (siRNAs) targeting the same region of a target-messenger RNA can have widely different efficacies. In efforts to better understand the
Limiting Ago protein restricts RNAi and microRNA biogenesis during early development in Xenopus laevis.
Lund, E; Sheets, MD; Imboden, SB; Dahlberg, JE
Genes & Development null
Identification of distinct miRNA target regulation between breast cancer molecular subtypes using AGO2-PAR-CLIP and patient datasets.
Farazi, TA; Ten Hoeve, JJ; Brown, M; Mihailovic, A; Horlings, HM; van de Vijver et al.
Genome Biology null
A Maxwell Burroughs et al.
Genome research, 20(10), 1398-1410 (2010-08-20)
Animal microRNA sequences are subject to 3' nucleotide addition. Through detailed analysis of deep-sequenced short RNA data sets, we show adenylation and uridylation of miRNA is globally present and conserved across Drosophila and vertebrates. To better understand 3' adenylation function
Fengying Guo et al.
Cytogenetic and genome research, 162(1-2), 10-27 (2022-03-31)
Long noncoding RNA FOXD2 adjacent opposite strand RNA1 (FOXD2-AS1) plays an oncogenic role in various cancers, including gastric cancer (GC). However, the function of FOXD2-AS1 in regulating radiosensitivity of GC cells and its underlying molecular mechanisms have not been elucidated.
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Related Content

RNA-Binding Protein Immunoprecipitation

All eukaryotic organisms require tight regulation of gene expression for complex processes such as development, differentiation, cell specification, and responses to environmental stimuli. Many genes are regulated post-transcriptionally, in addition to transcriptional mechanisms of gene regulation. RNA-binding proteins (RBPs) are essential for post-transcriptional gene regulation, linking transcription and translation in many processes including transcription, splicing, export, rate of translation and turnover. In all of these events, RBPs coordinate regulation of the amount of protein produced from mRNA transcripts.

Global Trade Item Number

SKUGTIN
07-590-25UG04054839342516

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