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Merck

07-818

Anti-phospho-EGFR (Tyr1086) Antibody

Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-phospho-EGFR (Tyr1086) Antibody, Upstate®, from rabbit

biological source

rabbit

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

NCBI accession no.

NM_005228.3
NM_201282.1
NM_201283.1
NM_201284.1

UniProt accession no.

P00533

shipped in

dry ice

target post-translational modification

phosphorylation (pTyr1086)

Quality Level

100

Gene Information

human ... EGFR(1956)

Analysis Note

Routinely evaluated by immunoblot.

Application

Anti-phospho-EGFR (Tyr1086) Antibody detects level of phospho-EGFR (Tyr1086) & has been published & validated for use in WB.
Immunoblot Analysis: A 1:1000 dilution of this lot detected phospho-EGFR (Tyr1086) in RIPA lysates from EGF stimulated A431 cells.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors

Biochem/physiol Actions

Predicted to cross-react with mouse and rat based on sequence homology.
Recognizes phospho-EGFR (Tyr1086)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

185 kDa

Immunogen

Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-EGFR (Tyr1086)

Other Notes

Replaces: 04-340

Physical form

ImmunoAffinity Purified

Preparation Note

Stable for 2 years at -20°C from date of shipment.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Changes in retinal cell fate induced by overexpression of EGF receptor
Lillien, L.
Nature, 377, 158-162 (1995)
Polymorphonuclear leukocytes-mediated lysis of A431 cells induced by IgG1 mouse anti-epidermal growth factor receptor monoclonal antibodies.
Kawamoto, T, et al.
In Vitro Cellular & Developmental Biology, 28A, 782-786 (1992)
G R Johnson et al.
The Journal of biological chemistry, 268(4), 2924-2931 (1993-02-05)
The COOH-terminal half of the amphiregulin (AR) molecule has sequence homology to epidermal growth factor (EGF). The ability of AR to elicit in vivo phosphorylation of the EGF receptor (EGFR) and p185erbB2 was studied in four human epithelial cell lines
Cytotoxic properties of DAB486EGF and DAB389EGF, epidermal growth factor (EGF) receptor-targeted fusion toxins.
Shaw, J P, et al.
The Journal of Biological Chemistry, 266, 21118-21124 (1991)

Global Trade Item Number

SKUGTIN
07-81804053252730337

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