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Merck

07-823

Anti-phospho eIF4E (Ser209) Antibody

Upstate®, from rabbit

Synonym(s):

eukaryotic translation initiation factor 4E, CBP, EIF4EL1, EIF4F

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-phospho eIF4E (Ser209) Antibody, Upstate®, from rabbit

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

rat (100% homologous), mouse (100% homologous), rabbit (100% homologous)

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NM_001968.2

UniProt accession no.

P06730

shipped in

dry ice

target post-translational modification

phosphorylation (pSer209)

Quality Level

100

Gene Information

human ... EIF4E(1977)

Application

Anti-phospho eIF4E (Ser209) Antibody is an antibody against phospho eIF4E (Ser209) for use in WB.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling

RNA Binding Protein (RBP)

Biochem/physiol Actions

Recognizes eIF4E phosphorylated on Ser209.

Analysis Note

Routinely evaluated by immunoblot.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

25 kDa

Immunogen

KLH conjugated synthetic peptide corresponding to amino acids surrounding Ser209 of human eIF4E. Sequence conserved in mouse and rat.

Physical form

ImmunoAffinity Purified

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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A J Waskiewicz et al.
Molecular and cellular biology, 19(3), 1871-1880 (1999-02-18)
Eukaryotic translation initiation factor 4E (eIF4E) binds to the mRNA 5' cap and brings the mRNA into a complex with other protein synthesis initiation factors and ribosomes. The activity of mammalian eIF4E is important for the translation of capped mRNAs
Phosphorylation of eukaryotic initiation factor 4E markedly reduces its affinity for capped mRNA
Scheper, Gert C, et al
The Journal of Biological Chemistry, 277, 3303-3309 (2002)
B Joshi et al.
The Journal of biological chemistry, 270(24), 14597-14603 (1995-06-16)
Initiation factor 4E (eIF-4E) binds to the m7GTP-containing cap of eukaryotic mRNA and facilitates the entry of mRNA into the initiation cycle of protein synthesis. eIF-4E is a phosphoprotein, and the phosphorylated form binds to mRNA caps 3-4-fold more tightly
Phosphorylation of eukaryotic initiation factor 4E (eIF4E) at Ser209 is not required for protein synthesis in vitro and in vivo
McKendrick, L, et al
European Journal of Biochemistry, 268, 5375-5385 (2001)
A Flynn et al.
The Journal of biological chemistry, 270(37), 21684-21688 (1995-09-15)
Ser-53 has previously been considered the major phosphorylation site in eukaryotic initiation factor (eIF)-4E, and this appeared to be supported by studies using a S53A mutant. Recently, however, several lines of evidence have indicated that Ser-53 might not be the
View All Related Papers

Related Content

RNA-Binding Protein Immunoprecipitation

All eukaryotic organisms require tight regulation of gene expression for complex processes such as development, differentiation, cell specification, and responses to environmental stimuli. Many genes are regulated post-transcriptionally, in addition to transcriptional mechanisms of gene regulation. RNA-binding proteins (RBPs) are essential for post-transcriptional gene regulation, linking transcription and translation in many processes including transcription, splicing, export, rate of translation and turnover. In all of these events, RBPs coordinate regulation of the amount of protein produced from mRNA transcripts.

Global Trade Item Number

SKUGTIN
07-82304053252403798

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