Key Documents
biological source
rabbit
Quality Level
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat (95% sequence homology)
species reactivity (predicted by homology)
porcine (based on 95% sequence homology), dog (based on 95% sequence homolgy), horse (based on 95% sequence homology)
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
UniProt accession no.
shipped in
wet ice
General description
Tks4 (gene symbol SH3PXD2B) is a novel adaptor protein; also known as SH3 and PX domain-containing protein 2B, (Courtneidge, 2005). It is closely related to Tks5 (gene symbol SH3PXD2A) and like Tks5, appears to play a role in the formation and function of podosome/invadopodia based on studies of Src-transformed fibroblasts (Buschman, 2009). Additionally, Tks4 may be involved in the recruitment of MT1-MMP and ECM degradation.
Tks4 is broadly expressed and localizes in the cytoplasm of the podosome.
Tks4 is broadly expressed and localizes in the cytoplasm of the podosome.
~ 130 kDa
Immunogen
Epitope: a. a. 257-477
Recombinant protein containing amino acids 257-477 of mouse Tks4.
Application
Anti-mouse Tks4 Antibody is an antibody against mouse Tks4 for use in WB, IC & IP.
Research Category
Signaling
Cell Structure
Signaling
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeletal Signaling
Cytoskeleton
Cytoskeletal Signaling
Biochem/physiol Actions
Predicted to cross react with rat (96%), horse (88%), canine (87%) and bovine (87%) based on sequence homology. Reactivity with other species has not been tested.
The antibody recognizes mouse Tks4.
Physical form
Format: Purified
Protein A purified
Purified in 0.1M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% NaN3.
Preparation Note
Maintain at 2-8°C for up to 1 year from date of receipt.
Analysis Note
Control
NIH3T3 cell lysate.
NIH3T3 cell lysate.
Evaluated on a representative lot by Immunoprecipitation followed by Western blot on NIH3T3 cell lysate using Anti-mouse Tks4.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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The SRC substrate Tks5, podosomes (invadopodia), and cancer cell invasion.
Courtneidge, S A, et al.
Cold Spring Harbor Symposia on Quantitative Biology, 70, 167-171 (2005)
Elena L Bolkhovitina et al.
International journal of molecular sciences, 23(15) (2022-07-29)
Vaccination protects against COVID-19 via the spike protein receptor-binding domain (RBD)-specific antibody formation, but it also affects the innate immunity. The effects of specific antibody induction on neutrophils that can cause severe respiratory inflammation are important, though not completely investigated.
Sarah Foerster et al.
Nature neuroscience, 27(8), 1545-1554 (2024-06-08)
In the mouse embryonic forebrain, developmentally distinct oligodendrocyte progenitor cell populations and their progeny, oligodendrocytes, emerge from three distinct regions in a spatiotemporal gradient from ventral to dorsal. However, the functional importance of this oligodendrocyte developmental heterogeneity is unknown. Using
Matthew D Buschman et al.
Molecular biology of the cell, 20(5), 1302-1311 (2009-01-16)
Metastatic cancer cells have the ability to both degrade and migrate through the extracellular matrix (ECM). Invasiveness can be correlated with the presence of dynamic actin-rich membrane structures called podosomes or invadopodia. We showed previously that the adaptor protein tyrosine
Antoine Oprescu et al.
Scientific reports, 12(1), 6255-6255 (2022-04-17)
Bone marrow megakaryocytes (MKs) undergo a maturation involving contacts with the microenvironment before extending proplatelets through sinusoids to deliver platelets in the bloodstream. We demonstrated that MKs assemble linear F-actin-enriched podosomes on collagen I fibers. Microscopy analysis evidenced an inverse
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