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09-290

Sigma-Aldrich

Anti-phospho-AMPK1 (Thr174) Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

5′-AMP-activated protein kinase, catalytic alpha-1 chain, AMP -activate kinase alpha 1 subunit, AMP-activated protein kinase, catalytic, alpha-1, AMPK alpha 1, AMPK alpha-1 chain, protein kinase, AMP-activated, alpha 1 catalytic subunit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, canine

species reactivity (predicted by homology)

chicken, mouse, rat, bovine, dog

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NP_006242

UniProt accession no.

Q13131

shipped in

wet ice

target post-translational modification

phosphorylation (pThr174)

Gene Information

human ... PRKAA1(5562)
mouse ... Hsf3(245525)

General description

60 kDa
AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. The Alpha subunit has at least two isoforms (alpha 1 and alpha 2) that differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 174 in the activation loop of the alpha subunit is a key determinant of AMPK activity.

Immunogen

KLH-conjugated linear peptide corresponding to phosphorylated human AMPK1 (Thr174).

Application

Anti-phospho-AMPK1 (Thr174) Antibody detects level of phospho-AMPK1 (Thr174) & has been published & validated for use in WB, IH.
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases
Western Blot (SNAP ID) Analysis: 2 µg/mL of this antibody detected AMPK1 phosphorylated at Thr174 in HEK293 cell lysate (see page 2).

Biochem/physiol Actions

This antibody recognizes phosphorylated AMPK1 at Thr174.

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Maintain refrigerated at 2-8°C for up to 12 months.

Analysis Note

Control
Lambda phosphatase treated and untreated Hek293 cell lysate
Evaluated by Western Blot in Lambda phosphatase treated and untreated HEK293 cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected AMPK1 phosphorylated at Thr174 in Lambda phosphatase treated and untreated HEK293 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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