Anti-phospho-AS160 (Ser666) Antibody

160 kDa

AS160 (Akt Substrate of 160 kDa, TBC1D4) is a Rab-GAP protein that regulates the trafficking of GLUT4 (glucose transporter 4) to the plasma membrane. AS160 is known to be phosphorylated on at least 8 residues following stimulation from IGF-1, EGF, PMA, and AICAR (Chen, S., 2008). Thr642 is phosphorylated by Akt in response to IGF-1, while Ser341 and Thr642 of AS160 are phosphorylated by RSK (p90 ribosomal S6 kinase) in response to PMA. Ser588 has been shown to be phosphorylated by AMPK in vitro, though other kinases might be responsible as well. The phosphorylation site, Ser666, does not contain the Akt consensus sequence. Its phosphorylation is suppressed by AICAR and by combined mutations of TOS (mTOR signaling)-like sequence (FEMDI) and rapamycin (Geraghty, K.M., 2007). Additionally, its phosphorylation is markedly increased in response to IGF-1 and this phosphorylation is blocked in response to the PI3 Kinase inhibitor LY294002 (Geraghty, K.M., 2007).

Epitope: Phosphorylated Ser666

Synthetic peptide corresponding to amino acids including and surrounding phosphorylated Ser666 of human AS160.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

This Anti-phospho-AS160 (Ser666) Antibody is validated for use in WB for the detection of phospho-AS160 (Ser666).

Other species have not been tested, but immunogen shares 79% homology with mouse and 90% homology with cow, horse, and dog.

This antibody detected AS160 when phosphorylated on Ser666.

Affinity purified

Affinity purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8ºC from date of receipt.

Evaluated by western blot on untreated and IGF1 treated HEK293 cell lysate.

Western Blot Analysis:
1:250 dilution of this antibody was used to detect AS160 phosphorylated on Ser666 in IGF1-treated HEK293 cell lysate.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.