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09-495

Anti-phospho LKB1 (Ser431) Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Renal carcinoma antigen NY-REN-19, Serine/threonine-protein kinase LKB1, polarization-related protein LKB1, serine/threonine kinase 11, erine/threonine kinase 11 (Peutz-Jeghers syndrome), serine/threonine protein kinase 11

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
horse, mouse
Application:
WB
Citations:
1
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biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

horse, mouse

species reactivity (predicted by homology)

equine (based on 100% sequence homology), human (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

NP_035622

UniProt accession no.

Q9WTK7

shipped in

wet ice

target post-translational modification

phosphorylation (pSer431)

Gene Information

human ... STK11(6794)
mouse ... Stk11(20869)

General description

LKB1 is a serine-threonine protein kinase and member of the calcium calmodulin family. LKB1 acts as a tumor suppressor and plays an important role in the regulation of many cellular processes. It is expressed in all tissues including pancreatic, liver, testicular, cardiac, and skeletal muscle tissue. Mutations in LKB1 result from unregulated cell growth and tumor formation that are associated with Peutz-Jehgers syndrome.
~ 55 kDa observed

Immunogen

Epitope: Phosphorylated Ser431
KLH-conjugated linear peptide corresponding to human LKB1 when phosphorylated at Ser431.

Application

This Anti-phospho LKB1 (Ser431) Antibody is validated for use in WB for the detection of phospho LKB1 (Ser431).
Western Blot (Snap i.d.) Analysis: 0.5 µg/mL from a previous lot detected LKB1 on 10 µg of NIH/3T3 treated with forskolin lysate.

Biochem/physiol Actions

This antibody recognizes LKB1 when phosphorylated at Ser431.

Analysis Note

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected LKB1 on 10 µg of NIH/3T3 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dania C Liemburg-Apers et al.
Journal of cell science, 129(23), 4411-4423 (2016-11-02)
Mitochondria play a central role in cellular energy production, and their dysfunction can trigger a compensatory increase in glycolytic flux to sustain cellular ATP levels. Here, we studied the mechanism of this homeostatic phenomenon in C2C12 myoblasts. Acute (30 min) mitoenergetic