Anti-phospho-Histone H3(Ser10) Antibody

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

~17 kDa

Epitope: phosphoSer10

The immunogen was MAPS conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser10 of human histone cluster 1, H3B.

ELISA:
This antibody was demonstrated by an outside laboratory to be suitable for ELISA.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-phospho-Histone H3(Ser10) Antibody (Rabbit Polyclonal Antibody) validated in WB, ELISA, IHC to detect phospho-Histone H3(Ser10) also known as H3S10P, Histone H3 (phospho S10).

Not tested in other species.

This antibody recognizes histone H3 phosphorylated at Ser10.

Format: Purified

Protein G Chromatography

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
Colcemid treated HeLa acid extracts

Western Blot Analysis:
This antibody detected phosphohistone H3 in 10 µg of colcemid-treated HeLa acid extract.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-1093

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.