Anti-RING finger protein 8 Antibody

RING Finger protein 8 (RNF8) is DNA binding protein belonging to the RING (Really Interesting New Gene) finger group of proteins. This family is characterized by a RING finger domain containing a Cys3HisCys4 amino acid motif capable of binding two zinc cations. RNF8 contains two domains of interest, the characteristic RING finger motif in the C-terminus and a FHA domain within the N-terminus, and is a critical E3 ubiquitin-protein ligase involved in the assembly of repair proteins to damaged DNA sites. After DNA double-strand breaks, RNF8 is recruited by ATM-phosphorylated MDC1 to the damage sites, where it functions to mediate H2A and H2AX ubiquitination, which in turn promotes TPR53BPI formation and the ionizing radiation-induced foci (IRIF) of BRCA1. RNF8 may also play regulatory roles in cytokinesis, correct exiting from mitosis, and RXRA-mediated transcriptional activity.

~ 58 kDa observed. Two different isoforms have been described. Isoform 1: 55.5 kDa and Isoform 2: 11 kDa

Linear peptide corresponding to amino acids 135-148 of human RING finger protein 8.

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

Cell Cycle, DNA Replication & Repair

Use Anti-RING finger protein 8 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect RING finger protein 8 also known as C3HC4-type zinc finger protein.

Western Blot (SNAP ID) Analysis: 1 µg/mL of a representative lot of this antibody detected RING finger protein 8 on 10 µg of U2OS nuclear extract.

Immunocytochemistry Analysis: 1:500 dilution of a representative lot this antibody detected RING finger protein 8 in NIH/3T3 and A431 cells.

This antibody recognizes RING finger protein 8.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
U2OS nuclear extract

Evaluated by Western Blot in U2OS nuclear extract.

Western Blot Analysis: 0.1 µg/mL of this antibody detected RING finger protein 8 on 10 µg of U2OS nuclear extract.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.