Anti-DDX3X Antibody

DDX3X, DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked, is a member of the DEAD box family of proteins. Characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), DEAD box proteins are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX3X is an ATP-dependent RNA helicase and acts as a co-factor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. DDX3X is also involved in HIV-1 replication and interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.

~73 kDa

KLH-conjugated synthetic linear peptide corresponding to DDX3X.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Binding Protein (RBP)

Use Anti-DDX3X Antibody (Rabbit Polyclonal Antibody) validated in WB to detect DDX3X also known as ATP-dependent RNA helicase DDX3X, DEAD box protein 3 X-chromosomal.

This antibody detects RNA-binding protein DDX3X.

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
A431 cell lysate

Evaluated by western blot on A431 cell lysate.

Western Blot Analysis: 1:1000 dilution of this antibody detected DDX3X in A431 cell lysate.

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