09-872
Anti-Methylcytosine dioxygenase TET1 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
tet oncogene 1, Ten-eleven translocation 1 gene protein, CXXC-type zinc finger protein 6, methylcytosine dioxygenase TET1, CXXC finger 6, ten-eleven translocation-1, Leukemia-associated protein with a CXXC domain
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse
technique(s)
ChIP: suitable (ChIP-seq)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
Gene Information
human ... TET1(80312)
General description
Methylcytosine dioxygenase TET1 (mTET1) is a member of the TET family and contains a characteristic CXXC-type zinc finger domain. mTET1 binds methylcytosine (5mC), a minor, mammalian DNA base necessary to development and retrotransposon silencing, and acts as a catalyst to induce the conversion of 5mC to 5-hydromethylcytosine (hmC or 5hmC), a mammalian DNA base thought to be involved in chromatin structure and in the development of fetal brain, heart and lung tissues. Recent studies indicate that mTET1 regulates the levels of 5hmC, thereby influencing patterns in DNA methylation.
~220 kDa observed.
Uncharacterized bands may be seen at ~32, 68, and 70 kDa
Uncharacterized bands may be seen at ~32, 68, and 70 kDa
Immunogen
Epitope: Near the C-terminus
KLH-conjugated linear peptide corresponding to mouse Methylcytosine dioxygenase TET1 near the C-terminus.
Application
Anti-Methylcytosine dioxygenase TET1 Antibody is a Rabbit Polyclonal Antibody for detection of Methylcytosine dioxygenase TET1 also known as tet oncogene 1 & has been validated in WB, ChIP-seq.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histone Modifying Proteins
Histone Modifying Proteins
Western Blot (Snap i.d.) Analysis: 5 µg/mL from a previous lot detected Methylcytosine dioxygenase TET1 in 10 µg of mouse embryonic stem cell lysate.
Biochem/physiol Actions
Other homologies: Rat (66% sequence homology), Human (61% sequence homology).
This antibody recognizes methylcytosine dioxygenase TET1 near the C-terminus.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse embryonic stem cell lysate
Mouse embryonic stem cell lysate
Evaluated by Western Blot in mouse embryonic stem cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Methylcytosine dioxygenase TET1 in 10 µg of mouse embryonic stem cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Methylcytosine dioxygenase TET1 in 10 µg of mouse embryonic stem cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Suppression of TET1-dependent DNA demethylation is essential for KRAS-mediated transformation.
Wu, BK; Brenner, C
Cell Reports null
Pietro Vella et al.
Molecular cell, 49(4), 645-656 (2013-01-29)
O-linked N-acetylglucosamine (O-GlcNAc) transferase (Ogt) activity is essential for embryonic stem cell (ESC) viability and mouse development. Ogt is present both in the cytoplasm and the nucleus of different cell types and catalyzes serine and threonine glycosylation. We have characterized
Dynamic hydroxymethylation of deoxyribonucleic acid marks differentiation-associated enhancers.
Serandour, Aurelien A, et al.
Nucleic Acids Research (2012)
Naoki Okashita et al.
Development (Cambridge, England), 141(2), 269-280 (2013-12-18)
Ten-eleven translocation (TET) proteins oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). 5fC and 5caC can be excised and repaired by the base excision repair (BER) pathway, implicating 5mC oxidation in active DNA demethylation. Genome-wide DNA methylation
Alain R Weber et al.
Nature communications, 7, 10806-10806 (2016-03-05)
Cytosine methylation in CpG dinucleotides is an epigenetic DNA modification dynamically established and maintained by DNA methyltransferases and demethylases. Molecular mechanisms of active DNA demethylation began to surface only recently with the discovery of the 5-methylcytosine (5mC)-directed hydroxylase and base
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