Anti-ALKBH3 Antibody

ABH3 is a member of the Fe(II)/2OG-dependent dioxygenase superfamily, which couples substrate oxidation by converting 2-oxoglutarate (2OG) into succinate and CO2. In humans, ABH3 plays a critical role in alkylated DNA/RNA damage repair and in cancer therapy. While ABH3 may repair alkylated RNA, it demonstrates greater preference for the repair of single- over double-stranded alkylated DNA through oxidative demethylation of 1-methyladenine and 3-methylcytosine.

~35 kDa observed

Other homologies Orangutan 98% and Rhesus Monkey 97% sequence homology.

This antibody recognizes the N-terminus of ALKBH3.

Epitope: N-terminus

Histidine-tagged-recombinant protein corresponding to the the N-terminus of human ALKBH3.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Use Anti-ALKBH3 Antibody (rabbit polyclonal antibody) validated in WB to detect ALKBH3 also known as Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, Alkylated DNA repair protein alkB homolog 3, DEPC-1, Prostate cancer antigen 1.

Western Blot Analysis: A representative lot from an independent laboratory detected ALKBH3 in PC-3 and LNCap whole cell extract. (Dango, S., et al. (2011). Mol Cell. {Epub ahead of print.})

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Human prostate tissue lysate

Evaluated by Western Blot in human prostate tissue lysate.

Western Blot Analysis: A 1:5,000 dilution of this antibody detected ALKBH3 in human prostate tissue lysate.

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