14-3-3 Antagonist I, 2-5

Name: 14-3-3 Antagonist I, 2-5
Brand: MM

The 14-3-3 Antagonist I, 2-5 controls the biological activity of 14-3-3.

Synonym(s):

14-3-3 Antagonist I, 2-5, (S)-2-(2-(4-Azidobenzamido)acetamido)-3-((4-hydroxyphenethyl)amino)-3-oxopropyl-dihydrogen phosphate

Select a Size

All Photos(1)

About This Item

Empirical Formula (Hill Notation):

C₂₀H₂₃N₆O₈P

Molecular Weight:

506.41

UNSPSC Code:

12352200

Quality Level

100

Assay

≥90% (HPLC)

form

solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze
desiccated (hygroscopic)
protect from light

color

pale yellow to beige

solubility

DMSO: 50 mg/mL

shipped in

wet ice

storage temp.

−20°C

General description

A cell-permeable dipeptidyl-phosphoserine compound that targets 14-3-3 phosphopeptide binding site and effectively competes against 14-3-3 ligands, including Raf-1 and p53, binding to 14-3-3 (IC₅₀ = 2.6 µM against TMR-GG-RLSHpSSLPG binding to human 14-3-3ς). Shown to induce caspase-3 activation and apoptotic cell death in human lung adenocarcinoma A549 cultures (live cell population after 12 h 100 µM drug treatment = 30% of control). Stability study using A549 cell lysates (100 µM drug in 1 µg/ml lysate) reveals a dephosphorylation t_1/2 of ~ 6 hrs and no detectable proteolytic degradation up to 12 hrs.

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Wu, H., et al. 2010. Angew. Chem. Int. Ed.49, 6528.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service