100081
14-3-3 Antagonist I, 2-5
The 14-3-3 Antagonist I, 2-5 controls the biological activity of 14-3-3.
Synonym(s):
14-3-3 Antagonist I, 2-5, (S)-2-(2-(4-Azidobenzamido)acetamido)-3-((4-hydroxyphenethyl)amino)-3-oxopropyl-dihydrogen phosphate
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About This Item
Empirical Formula (Hill Notation):
C20H23N6O8P
Molecular Weight:
506.41
UNSPSC Code:
12352200
Assay:
≥90% (HPLC)
Form:
solid
Storage condition:
OK to freeze, desiccated (hygroscopic), protect from light
Quality Segment
assay
≥90% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated (hygroscopic), protect from light
color
pale yellow to beige
solubility
DMSO: 50 mg/mL
shipped in
wet ice
storage temp.
−20°C
General description
A cell-permeable dipeptidyl-phosphoserine compound that targets 14-3-3 phosphopeptide binding site and effectively competes against 14-3-3 ligands, including Raf-1 and p53, binding to 14-3-3 (IC50 = 2.6 µM against TMR-GG-RLSHpSSLPG binding to human 14-3-3ς). Shown to induce caspase-3 activation and apoptotic cell death in human lung adenocarcinoma A549 cultures (live cell population after 12 h 100 µM drug treatment = 30% of control). Stability study using A549 cell lysates (100 µM drug in 1 µg/ml lysate) reveals a dephosphorylation t1/2 of ~ 6 hrs and no detectable proteolytic degradation up to 12 hrs.
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Wu, H., et al. 2010. Angew. Chem. Int. Ed.49, 6528.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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