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Merck

1.01865

Readybag® Half Fraser Broth with Supplements

acc. ISO 11290, 62 g, irradiated, For the primary selective enrichment of Listeria sp.

Synonym(s):

Demi Fraser broth, Fraser broth primary enrichment, Fraser Broth, Fraser Listeria selective enrichment Broth

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About This Item

NACRES:
NA.74
UNSPSC Code:
41106212

Key Documents

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agency

APHA, ISO 11133, ISO 11290

form

medium granules (dehydrated (DCM))

manufacturer/tradename

ReadyBag®

technique(s)

microbiological culture: suitable

pH

7.0-7.4 (25 °C, 55 g/L in H2O)

solubility

55 g/L

application(s)

food and beverages
microbiology
pathogen testing

storage temp.

15-25°C

suitability

Listeria spp.

Quality Level

200

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General description

Half FRASER (Demi FRASER) broth has high nutrient content and the large buffer capacity that creates optimum growth conditions for Listeria. Lithium chloride, nalidixic acid, and acriflavine hydrochloride largely inhibit the growth of accompanying bacteria. Esculin and ammonium iron (III) citrate enables the detection of the β-D-glucosidase activity by the formation of a black complex of esculetin with iron (III) ions. However, this reaction is not exclusive to Listeria sp. and hence every primary and secondary enrichment in FRASER broth has to be sub-cultured on selective plating media.

Application

Half FRASER (Demi FRASER) broth with supplements is used for the primary selective enrichment of Listeria sp. from food and animal feed as well as from environmental samples and other materials.

Legal Information

READYBAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Articles

Types of Media in Microbiology

The different types of culture media, that are used to grow microorganisms in the laboratory for quality control, are classified by several criteria, such as consistency, composition, or selectivity.

Protocols

Standards for Detection and Enumeration of Listeria monocytogenes and Listeria spp. in the food chain

Discover the revised EN ISO 11290-1 and EN ISO 11290-2 standards and gain insights into updated detection methods for Listeria monocytogenes and Listeria spp., including enumeration in food, animal feed, and environmental samples. Explore culture media solutions.

Understanding Listeria: Guide to Listeria Monocytogenes and Its Impact on Food Safety

Ensure food safety with our robust Listeria monocytogenes testing methods, including biochemical tests, chromogenic media, and rapid detection methods.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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