1.05548
HPTLC aluminum sheets, Silica gel 60 F₂₅₄
pkg of 25 sheets, sheet L × W 20 cm × 20 cm, aluminum support
Synonym(s):
HPTLC F₂₅₄ Silica Gel Plates
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About This Item
NACRES:
NB.21
UNSPSC Code:
41115711
Quality Level
material
aluminum support, silica gel 60 matrix
feature
binder Organic Polymer, fluorescent indicator
packaging
pkg of 25 sheets
technique(s)
thin layer chromatography (TLC): suitable
layer thickness
200 μm
plate L × W
20 cm × 20 cm
sheet L × W
20 cm × 20 cm
particle size
5-6 μm
pore size
60 Å medium pore diameter
storage temp.
2-30°C
General description
25 Aluminium sheets 20 x 20 cm
Application
- Novel Validated HPTLC Method for the Analysis of Two Binary Mixtures Containing Tamsulosin Hydrochloride with Antimuscarinic Agents.: This study presents a novel HPTLC method using aluminum sheets and Silica gel 60 F₂₅₄ for analyzing binary mixtures of Tamsulosin Hydrochloride with antimuscarinic agents. This method ensures accurate and efficient separation of the components (El-Kimary et al., 2018).
- Development and Validation of a High-Performance Thin-Layer Chromatographic Method for the Simultaneous Determination of Two Binary Mixtures Containing Ketorolac Tromethamine with Phenylephrine Hydrochloride and with Febuxostat.: This research developed a validated HPTLC method on aluminum sheets coated with Silica gel 60 F₂₅₄ for simultaneous determination of Ketorolac Tromethamine with Phenylephrine Hydrochloride and with Febuxostat, showcasing high accuracy and precision (El Yazbi et al., 2016).
- HPTLC Determination of Three Gliptins in Binary Mixtures with Metformin.: The article demonstrates the use of HPTLC aluminum sheets with Silica gel 60 F₂₅₄ for determining three gliptins in binary mixtures with Metformin, emphasizing the method′s efficiency and reliability (El-Kimary et al., 2016).
- Validated HPLC and HPTLC methods for simultaneous determination of colchicine and khellin in pharmaceutical formulations.: This paper details validated HPTLC methods on aluminum sheets and Silica gel 60 F₂₅₄ for simultaneous determination of colchicine and khellin, providing a robust and accurate analytical approach (Hadad et al., 2013).
Analysis Note
Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 30 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
17 - 22
- Ceres Black G, colour test, lipophile: 38 - 43
- ceres Violet brn, colour test, lipophile: 58 - 64
separation number
- colourtest, lipophile:
≥ 10.0
B) Steroid test
hRf-values
- hydrocortisone, steroid test:
23 - 29
- Reichstein S, steroid test: 36 - 45
- methyltestosterone, steroid test: 43 - 52
separation number
- steroid test:
≥ 10.5
Typical value determined on a conditioned sheet
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 30 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
17 - 22
- Ceres Black G, colour test, lipophile: 38 - 43
- ceres Violet brn, colour test, lipophile: 58 - 64
separation number
- colourtest, lipophile:
≥ 10.0
B) Steroid test
hRf-values
- hydrocortisone, steroid test:
23 - 29
- Reichstein S, steroid test: 36 - 45
- methyltestosterone, steroid test: 43 - 52
separation number
- steroid test:
≥ 10.5
Typical value determined on a conditioned sheet
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)
Other Notes
Replaces: 5548-7; 5548
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