1.07627
Pseudomonas CFC Selective Supplement
for Pseudomonas spp., pkg of 10 vials, for the preparation of Pseudomonas CFC Selective Agar
Synonym(s):
Pseudomonas Selective Supplement
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About This Item
NACRES:
NA.74
UNSPSC Code:
41106212
Quality Level
form
solid
composition
Cephalothin, 25 mg per vial , Cetrimide, 5 mg per vial , Fucidin, 5 mg per vial
packaging
pkg of 10 vials
technique(s)
microbiological culture: suitable
pH
6.2-6.8 (20 °C, 26.2 g/L in H2O)
solubility
26.6 g/L
application(s)
food and beverages
microbiology
compatibility
for use with Pseudomonas CFC/CN agar (base) acc. ISO 13720 and ISO 16266 (1.07620)
storage temp.
2-8°C
suitability
Pseudomonas spp.
General description
The Pseudomonas CFC Selective Supplement is a mixture of 3 different inhibitors in lyophilized form. Cetrimide, Fucidin, and Cephalothin inhibit the accompanying Gram-positive and Gram-negative flora.
Application
Pseudomonas CFC Selective Supplement is an additive for the preparation of GranuCult® prime Pseudomonas CFC/CN Agar (Base) (1.07620) for the detection and enumeration of Pseudomonas spp. from food and animal feed.
Analysis Note
Recovery on test medium
- Pseudomonas aeruginosa ATCC 27853 (WDCM 00025):
≥ 70 %
- Pseudomonas putida ATCC 12633 (WDCM 00117): ≥ 70 %
- Pseudomonas fluorescens ATCC 13525 (WDCM 00115): ≥ 70 %
- Pseudomonas fragi ATCC 27362: ≥ 70 %
- Pseudomonas fragi ATCC 4973 (WDCM 00116): ≥ 70 %
- Burkholderia cepacia ATCC 17759: ≥ 70 %
- Proteus mirabilis ATCC 14153: ≤ 0.01 %
- Staphylococcus aureus ATCC 25923 (WDCM 00034): ≤ 0.01 %
Colonies
- Pseudomonas aeruginosa ATCC 27853 (WDCM 00025):
greenish
- Pseudomonas putida ATCC 12633 (WDCM 00117): greenish
- Pseudomonas fluorescens ATCC 13525 (WDCM 00115): greenish
- Pseudomonas fragi ATCC 27362: milky
- Pseudomonas fragi ATCC 4973 (WDCM 00116): milky
- Burkholderia cepacia ATCC 17759: milky
Incubation: 44 hrs.; 25 °C; aerobic;
Reference: blood-agar (24-48 hrs.; 30 °C; aerobic).
Supplement tested in Pseudomonas selektiv agar (base) art. no. 1.07620.
- Pseudomonas aeruginosa ATCC 27853 (WDCM 00025):
≥ 70 %
- Pseudomonas putida ATCC 12633 (WDCM 00117): ≥ 70 %
- Pseudomonas fluorescens ATCC 13525 (WDCM 00115): ≥ 70 %
- Pseudomonas fragi ATCC 27362: ≥ 70 %
- Pseudomonas fragi ATCC 4973 (WDCM 00116): ≥ 70 %
- Burkholderia cepacia ATCC 17759: ≥ 70 %
- Proteus mirabilis ATCC 14153: ≤ 0.01 %
- Staphylococcus aureus ATCC 25923 (WDCM 00034): ≤ 0.01 %
Colonies
- Pseudomonas aeruginosa ATCC 27853 (WDCM 00025):
greenish
- Pseudomonas putida ATCC 12633 (WDCM 00117): greenish
- Pseudomonas fluorescens ATCC 13525 (WDCM 00115): greenish
- Pseudomonas fragi ATCC 27362: milky
- Pseudomonas fragi ATCC 4973 (WDCM 00116): milky
- Burkholderia cepacia ATCC 17759: milky
Incubation: 44 hrs.; 25 °C; aerobic;
Reference: blood-agar (24-48 hrs.; 30 °C; aerobic).
Supplement tested in Pseudomonas selektiv agar (base) art. no. 1.07620.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
Certificates of Analysis (COA)
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