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1.10694

DEV TRYPTOPHAN BROTH

NutriSelect® Prime, according to ISO (6579), ISO (16654), ISO (21567), powder, suitable for microbiology

Synonym(s):

Tryptone Broth

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About This Item

NACRES:
NA.74
UNSPSC Code:
41106213
Manufacturer/tradename:
NutriSelect® Prime
Agency:
ISO (16654), ISO (21567), according to ISO (6579)
Ph:
7.5±0.2 (25 °C)
Form:
powder
Suitability:
Escherichia coli, coliforms
Application(s):
food and beverages, microbiology
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Quality Segment

agency

ISO (16654), ISO (21567), according to ISO (6579)

product line

GranuCult® plus

form

powder

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Prime

technique(s)

microbe id | specific enzyme detection: suitable, microbiological culture: suitable

color

beige

pH

7.5±0.2 (25 °C)

solubility

16 g/L

application(s)

food and beverages, microbiology

storage temp.

15-25°C

suitability

Escherichia coli, coliforms

General description

Tryptone/Tryptophan Broth is a selective culture medium used to detect indole production by microorganisms. It contains tryptone as a source of nitrogen, DL-tryptophan as the substrate for indole formation, and sodium chloride to maintain osmotic balance. After incubation, indole production is detected by adding Kovacs’ indole reagent. The formation of a red ring in the upper layer indicates a positive reaction and supports the confirmation and identification of bacterial isolates.

Application

Tryptophan Broth is used for the confirmation and identification of microorganisms isolated from food, animal feed, water, and other materials. It is applied in microbiological testing workflows for organisms such as Escherichia coli, Salmonella, Shigella, and other bacteria where indole production is an important identification characteristic.

Analysis Note

Appearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25 °C): 7.0 - 7.4
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Indole formation (Escherichia coli ATCC 25922 (WDCM 00013)): +
Growth (Escherichia coli ATCC 11775 (WDCM 00090)): good to very good
Indole formation (Escherichia coli ATCC 11775 (WDCM 00090)): +
Growth (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): good to very good
Indole formation (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): -
Growth (Proteus mirabilis ATCC 14153): good to very good
Indole formation (Proteus mirabilis ATCC 14153): -
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Indole formation (Salmonella typhimurium ATCC 14028 (WDCM 00031)): -
Incubation:4 -6 hrs.; 35°C; aerobic.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

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