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1.16855

Superspher® 100 RP-18 endcapped (4µm) HPLC Columns

L x I.D. 125 mm x 4 mm, LiChroCART™ HPLC cartridge, manuCART™ holder required for use

Synonym(s):

TM=["Superspher"] C18 TM=["LICHROCART"] cartridge for HPLC

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52
Particle size:
4 μm
Matrix active group:
C18 (RP18), C18 bonding phase
Pore size:
100 Å pore size
Matrix:
fully porous particle
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Product Name

Superspher® 100 RP-18 endcapped LiChroCART™ 125-4, suitable for HPLC

material

stainless steel column

Quality Segment

agency

suitable for USP L1

product line

Superspher®

feature

endcapped

analyte functional class(es)

antibiotics, explosives, fat soluble vitamins, lipids, nutraceuticals and antioxidants, oils, pigments, sweeteners, water soluble vitamins

analyte chemical class(es)

PAHs, PCBs (aroclor identification), PFAS, aflatoxins, alcohols / fermentation, alcohols and aldehyde, aliphatic amines, alkaloids, amino acids, aromatic amines, cannabinoids as tms derivatives, carotenoids, catecholamines, coloring compounds, essential oils, esters, fatty acids, flavonoids, glycans, ketones, metabolized steroids, nitrosamines, nucleic acids, nucleosides, oligonucleotides, organic phosphates, peptides, pesticides, phenols, phospholipids, phthalate esters, preservatives, proteins, steroids (cholesteryl esters), sulfonamides

parameter

250 bar pressure, 60 °C max. temp.

technique(s)

HPLC: suitable, mass spectrometry (MS): suitable (Low - Medium)

column L × I.D.

12.5 cm × 4 mm

matrix

fully porous particle

matrix active group

C18 (RP18), C18 bonding phase

particle size

4 μm

pore size

100 Å pore size

operating pH range

2-7.5

separation technique

reversed phase

storage temp.

no temp limit

General description

HPLC cartridge
Superspher® RP-18 endcapped is a reliable and versatile traditionally produced spherical silica gel carriers with C-18 endcapped reversed phase properties. They are well suited for the separation of neutral, acidic and weak basic compounds. This high performance silica carrier with a particle size of 4µm yields an excellent pressure/separation performance ratio.This sorbent is packed into a LiChroCART 125-4 HPLC cartridge. The connection to the HPLC system is being accomplished by use of the manu-CART NT cartrige holder (1.51486).

Application


  • Chemometric approach for estimation of ropinirole hydrochloride: This study presents a chemometric approach for the development and validation of an HPLC method for estimating ropinirole hydrochloride using LiChrospher 100 RP-18 columns. The method shows robustness and efficiency for pharmaceutical analysis (Fatima et al., 2019).

  • Simultaneous quantification of Parkinson′s disease medications: An HPLC method using LiChrospher 100 RP-18 was validated for simultaneous quantification of levodopa, carbidopa, benserazide, and entacapone in complex matrices, proving its capability in pharmaceutical formulations (Wollmer & Klein, 2017).

  • Stability of THCA and (9)-THC in herbal cannabis: This research employed LiChrospher 100 RP-18 columns to determine the stability of THCA and Δ(9)-THC in herbal cannabis under different storage temperatures, important for forensic and pharmaceutical sciences (Taschwer & Schmid, 2015).

  • Stability indicating method for cilazapril: Researchers developed a rapid and simple stability-indicating HPLC method for cilazapril, using LiChrospher 100 RP-18, to assess drug stability in pharmaceuticals, compared to spectrophotometric methods, highlighting its precision and reliability (Paszun et al., 2012).


Analysis Note

Pressure: ≤ 80 bar
Theoretical Plates (N/m) (Anthracene): ≥ 100000
Symmetry (Anthracene): 0.8 - 1.4
Capacity factor (Anthracene): 3.5 - 5.5

Legal Information

LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
Superspher is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

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