1.19613
Superspher® 100 RP-18 (4 µm) HPLC Columns
HPLC sorbent
Synonym(s):
TM=["Superspher"] C18 sorbent for HPLC
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About This Item
UNSPSC Code:
41115709
NACRES:
SB.52
Particle size:
4 μm
Matrix active group:
C18 bonding phase
Pore size:
100 Å pore size
Matrix:
fully porous particle
Product Name
Superspher® 100 RP-18,
material
stainless steel column
agency
suitable for USP L1
product line
Superspher®
feature
endcapped: no
parameter
60 °C max. temp.
technique(s)
HPLC: suitable, mass spectrometry (MS): suitable (Low - Medium)
matrix
fully porous particle
matrix active group
C18 bonding phase
particle size
4 μm
pore size
100 Å pore size
operating pH range
2-7.5
bulk density
200‑400 kg/m3
separation technique
reversed phase
storage temp.
no temp limit
General description
Superspher® RP-18 is a reliable and versatile traditionally produced spherical silica gel carriers with C-18 reversed phase properties. They are well suited for the separation of neutral, acidic and weak basic compounds. This high performance silica carrier with a particle size of 4µm yields an excellent pressure/separation performance ratio.
Application
- Determination of retinol and retinyl esters in human plasma: This study utilized high-performance liquid chromatography with automated column switching and ultraviolet detection, highlighting the utility of the Superspher 100 RP-18 HPLC column in sensitive biochemical analyses (Hartmann et al., 2001).
- Enantioselective assay for ketoprofen glucuronidation: This study underscores the column′s capability in pharmacokinetic research, using HPLC to analyze chiral anti-inflammatory drugs and their metabolites in liver microsomes (Chakir et al., 1994).
- Rapid liquid chromatographic assay of glutathione in cultured cells: Employing the Superspher 100 RP-18 column, this research focused on rapid and effective glutathione quantification, demonstrating the column′s versatility in biomedicine (Leroy et al., 1993).
- Assay of chloramphenicol acetyl transferase by high-performance liquid chromatography: This application further exemplifies the adaptability of Superspher® 100 RP-18 in biotechnology, offering precise measurement techniques for enzymatic activity studies (Burzio et al., 1988).
Analysis Note
Application test: passes test
Pressure: 50 - 70 bar
Theoretical Plates (N/m) (Anthracene): ≥ 105000
Symmetry (Anthracene): 0.8 - 1.4
Capacity factor (Anthracene): 4.0 - 5.5
separation factor (Acetophenone/Phenol, selectivity test): 1.90 - 2.15
separation factor (Acetophenone/Cinnamic acid, selectivity test): 1.20 - 1.37
separation factor (1-Naphtol/Phenol, selectivity test): 3.80 - 4.30
capacity factor (Toluene): 17.0 - 22.0
Pressure: 50 - 70 bar
Theoretical Plates (N/m) (Anthracene): ≥ 105000
Symmetry (Anthracene): 0.8 - 1.4
Capacity factor (Anthracene): 4.0 - 5.5
separation factor (Acetophenone/Phenol, selectivity test): 1.90 - 2.15
separation factor (Acetophenone/Cinnamic acid, selectivity test): 1.20 - 1.37
separation factor (1-Naphtol/Phenol, selectivity test): 3.80 - 4.30
capacity factor (Toluene): 17.0 - 22.0
Legal Information
Superspher is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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