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1.19613

Superspher^® 100 RP-18

Name: Superspher<SUP>&#174;</SUP> 100 RP-18
Brand: MM

HPLC sorbent

Synonym(s):

TM=["Superspher"] C18 sorbent for HPLC

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About This Item

UNSPSC Code:

41115709

NACRES:

SB.52

eCl@ss:

32110501

Particle size:

4 μm

Matrix active group:

C18 (RP18), C18 bonding phase

Pore size:

100 Å pore size

Matrix:

fully porous particle

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Properties

Product Name

Superspher^® 100 RP-18,

material

stainless steel column

agency

suitable for USP L1

product line

Superspher^®

feature

endcapped: no

analyte functional class(es)

antibiotics, explosives, fat soluble vitamins, lipids, nutraceuticals and antioxidants, oils, pigments, sweeteners, water soluble vitamins

analyte chemical class(es)

PAHs, PCBs (aroclor identification), PFAS, aflatoxins, alcohols / fermentation, alcohols and aldehyde, aliphatic amines, alkaloids, amino acids, aromatic amines, cannabinoids as tms derivatives, carotenoids, catecholamines, coloring compounds, essential oils, esters, fatty acids, flavonoids, glycans, ketones, metabolized steroids, nitrosamines, nucleic acids, nucleosides, oligonucleotides, organic phosphates, peptides, pesticides, phenols, phospholipids, phthalate esters, preservatives, proteins, steroids (cholesteryl esters), sulfonamides

parameter

60 °C max. temp.

technique(s)

HPLC: suitable, mass spectrometry (MS): suitable (Low - Medium)

matrix

fully porous particle

matrix active group

C18 (RP18), C18 bonding phase

particle size

4 μm

pore size

100 Å pore size

operating pH range

2-7.5

bulk density

200‑400 kg/m³

separation technique

reversed phase

storage temp.

no temp limit

Description

General description

Superspher^® RP-18 is a reliable and versatile traditionally produced spherical silica gel carriers with C-18 reversed phase properties. They are well suited for the separation of neutral, acidic and weak basic compounds. This high performance silica carrier with a particle size of 4µm yields an excellent pressure/separation performance ratio.

Application

Determination of retinol and retinyl esters in human plasma: This study utilized high-performance liquid chromatography with automated column switching and ultraviolet detection, highlighting the utility of the Superspher 100 RP-18 HPLC column in sensitive biochemical analyses (Hartmann et al., 2001).

Enantioselective assay for ketoprofen glucuronidation: This study underscores the column′s capability in pharmacokinetic research, using HPLC to analyze chiral anti-inflammatory drugs and their metabolites in liver microsomes (Chakir et al., 1994).

Rapid liquid chromatographic assay of glutathione in cultured cells: Employing the Superspher 100 RP-18 column, this research focused on rapid and effective glutathione quantification, demonstrating the column′s versatility in biomedicine (Leroy et al., 1993).

Assay of chloramphenicol acetyl transferase by high-performance liquid chromatography: This application further exemplifies the adaptability of Superspher^® 100 RP-18 in biotechnology, offering precise measurement techniques for enzymatic activity studies (Burzio et al., 1988).

Analysis Note

Application test: passes test
Pressure: 50 - 70 bar
Theoretical Plates (N/m) (Anthracene): ≥ 105000
Symmetry (Anthracene): 0.8 - 1.4
Capacity factor (Anthracene): 4.0 - 5.5
separation factor (Acetophenone/Phenol, selectivity test): 1.90 - 2.15
separation factor (Acetophenone/Cinnamic acid, selectivity test): 1.20 - 1.37
separation factor (1-Naphtol/Phenol, selectivity test): 3.80 - 4.30
capacity factor (Toluene): 17.0 - 22.0

Legal Information

Superspher is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)