1.25085
Fractogel® EMD TMAE Hicap (M)
RoboColumn Fractogel® TMAE Hicap (M), volume 0.2 mL, prepacked column
Synonym(s):
RoboColumn Fractogel® TMAE Hicap (M), 0.2 ml
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About This Item
UNSPSC Code:
41115711
ligand
(Trimethylammoniumethyl)
material
polypropylene housing
product line
Fractogel®
form
prepacked column
parameter
≤8 bar pressure, ~180 cm/hr max. flow rate
column I.D. × L
5 mm × 10 mm
volume
0.2 mL
matrix active group
Methacrylate
mean particle size
40-90 μm
capacity
~180 mg, BSA binding capacity
application(s)
mAb
recombinant protein
vaccine development
separation technique
anion exchange
storage temp.
room temp
General description
5 x 10 mm
Features and Benefits
RoboColumn Fractogel® TMAE Hicap (M), 0.2 ml enables users to:
Due to the titration behavior of Fractogel® EMD TMAE Hicap (M) the ion exchange capacity can be used from pH 2 to 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD TMAE Hicap (M) when the pH of the buffer is about 1 unit above their isoelectric points (pl).
The strength of the binding depends on the following:
- Efficiently capture target protein and remove viruses, DNA, and endotoxins with its tentacle technology resulting in better production yields and high throughput
- Reduce operating costs and resin replacement with enduring durability - allowing for multiple cycles of column regeneration and sanitization
- Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution
Due to the titration behavior of Fractogel® EMD TMAE Hicap (M) the ion exchange capacity can be used from pH 2 to 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD TMAE Hicap (M) when the pH of the buffer is about 1 unit above their isoelectric points (pl).
The strength of the binding depends on the following:
- the buffer system
- pH value of the buffer which determines the surface charge of the protein
- the concentration of the counter ions
- the charge density on the support (protein binding capacity)
Legal Information
FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany
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