12660
Albumin, Bovine Serum, Fraction V, Modified Cohn, pH 5.2
Synonym(s):
Albumin, Bovine Serum, Fraction V, Modified Cohn, pH 5.2
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
Assay:
≥96% (SDS-PAGE)
Form:
solid
Impurities:
≤5% Moisture
description
Merck USA index - 14, 8468
Quality Segment
assay
≥96% (SDS-PAGE)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤5% Moisture
color
off-white
solubility
0.15 M sodium chloride: 10 mg/mL
cation traces
heavy metals: ≤50 ppm
shipped in
ambient
storage temp.
2-8°C
General description
Albumin, Bovine Serum, Fraction V, Modified Cohn, pH 5.2 is a native fatty acid profile that reflects endogenous lipids. It has a pH in the range of 4.8-5.4 in 150 mM NaCl (1%).
Bovine serum albumin (BSA), a non-glycosylated globular, α-helical protein, belongs to the serum albumin family. It consists of three domains with two sub-domains each and has 17-disulfide bonds.
Application
Albumin, Bovine Serum, Fraction V, Modified Cohn, pH 5.2 has been used as a blocking agent in western blotting. It has also been used as a component in phosphate-buffered saline (PBS) for diluting secondary antibodies and blocking in immunofluorescence to observe the localization of the Golgi apparatus.
Biochem/physiol Actions
Bovine Serum Albumin (BSA) is the most vital component of cell culture media. It aids in the differentiation of embryonic stem cells (hESC). It plays a key role in the transportation of drugs, hormones, and fatty acids. BSA is used in enzyme-linked immunosorbent assay (ELISA) as a blocking agent.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.