Human recombinant histone H2AZ-H2B dimers

Research Category
Epigenetics & Nuclear Function

Suitable for in vitro chromatin assembly and enzyme activity assays

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Human recombinant histone H2AZ-H2B dimers expressed in E Coli. as fusion proteins with either an N-terminal FLAG^® or 6xHis epitopes, and purified by Fast Protein Liquid Chromatography (FPLC).

Product Source: Human

The core histones, H2A, H2B, H3, and H4, form a central octameric structure that binds approximately 147 bp of DNA to form a nucleosome. The H2AZ histone variant replaces conventional H2A in a subset of nucleosomes and plays an essential role in transcriptional regulation, autosilencing, gene silencing, and genome stability. The effects of histone variants and histone modifications on chromatin structure and gene expression can be deciphered by applications such as chromatin immunoprecipiation (ChIP), which requires high quality chromatin and essential tools, such as histone proteins and appropriate enzymes to assemble chromatin in vitro.

In buffer containing 20 mM Tris-Cl pH 7.9, 1 mM EDTA, 1 M NaCl, 0.5 mM PMSF and 1 mM DTT

Stable at -80ºC for 1 year from date of receipt.

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