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Merck

14-306-M

GSK3β Protein, active, 10 µg

Active, N-terminal His6-tagged recombinant human GSK3β with an H350L mutation, for use in Kinase Assays.

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.26
eCl@ss:
32160405

Product Name

GSK3β Protein, active, 10 µg, Active, N-terminal His6-tagged recombinant human GSK3β with an H350L mutation, for use in Kinase Assays.

biological source

human

recombinant

expressed in baculovirus infected Sf21 cells

form

liquid

mol wt

Mw 51 kDa

composition

mercaptoethanol, 0.1-1%
saccharose, 5-10%

manufacturer/tradename

Upstate®

storage condition

(Tightly closed)

technique(s)

activity assay: suitable (kinase)

solubility

water: soluble

NCBI accession no.

NM_002093.2

UniProt accession no.

P49841

Quality Level

100

Gene Information

human ... GSK3B(2932)

Analysis Note

Routinely evaluated by phosphorylation of Phospho-Glycogen Synthase Peptide-2

Biochem/physiol Actions

Glycogen synthase kinase 3β (GSK3β) regulates glycogen metabolism via an insulin-dependent pathway. It participates in many signaling pathways including cellular processes such as cell cycle, cell proliferation, and inflammation. GSK3β mediated-negative regulation of insulin signaling pathway leads to type 2 diabetes. GSK3β functions as a tumor suppressor that represses neoplastic transformation in cells. It is an essential regulator of the Wnt/β-catenin signaling pathway. Abnormal regulation of GSK3β is associated with cardiovascular disease, bipolar disorder, and neurodegenerative diseases.

General description

Glycogen synthase kinase 3β (GSK3β) is a serine-threonine kinase that is present in most eukaryotes. The GSK3β gene is located on the human chromosome at 3q13.3. GSK3β is made up of an N-terminal β-sheet domain that is coupled to the C-terminal α-helical domain.

Research area: Cell Signaling

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Packaging

Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-306M when ordering the 250μg size.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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S Phukan et al.
British journal of pharmacology, 160(1), 1-19 (2010-03-25)
Glycogen synthase kinase-3 beta (GSK3beta) is a multifunctional serine/threonine kinase which was originally identified as a regulator of glycogen metabolism. It plays a key role in the regulation of numerous signalling pathways including cellular process such as cell cycle, inflammation
The alpha-isoform of glycogen synthase kinase-3 from rabbit skeletal muscle is inactivated by p70 S6 kinase or MAP kinase-activated protein kinase-1 in vitro.
Sutherland, C and Cohen, P
Febs Letters, 338, 37-42 (1994)
rystal structure of glycogen synthase kinase 3 beta: structural basis for phosphate-primed substrate specificity and autoinhibition
Dajani R, et al.
Cell, 105(6), 721-732 (2009)
Ethanol impairs insulin-stimulated neuronal survival in the developing brain: role of PTEN phosphatase
Xu, J., et al
The Journal of Biological Chemistry, 278, 26929-26937 (2003)
Jia Luo
Cancer letters, 273(2), 194-200 (2008-07-09)
Glycogen synthase kinase 3beta (GSK3beta), a multifunctional serine/threonine kinase found in all eukaryotes, had been initially identified as a key regulator of insulin-dependent glycogen synthesis. It is now known that GSK3beta functions in diverse cellular processes including proliferation, differentiation, motility
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Global Trade Item Number

SKUGTIN
14-306M04053252370427
14-30604053252409486

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