14-439-M

MAP Kinase 1/Erk1 Protein, active, 10 µg

Name: MAP Kinase 1/Erk1 Protein, active, 10 µg
Brand: MM

Active, recombinant, full-length, human MAP Kinase 1/Erk1 containing an N-terminal GST tag, for use in Kinase Assays.

Synonym(s):

ERT1, Extracellular signal-regulated kinase 2 (ERK-2), MAPK 1, Mitogen-activated protein kinase 1, p44 MAPK

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.32

eCl@ss:

32160405

Product Name

MAP Kinase 1/Erk1 Protein, active, 10 µg, Active, recombinant, full-length, human MAP Kinase 1/Erk1 containing an N-terminal GST tag, for use in Kinase Assays.

biological source

human

recombinant

expressed in E. coli

form

liquid

mol wt

M_w 69.9 kDa

manufacturer/tradename

Upstate^®

technique(s)

activity assay: suitable (kinase)

color

colorless

solubility

water: soluble

Analysis Note

routinely evaluated by phosphorylation of MBP

Biochem/physiol Actions

Extracellular-signal-regulated kinase 1 (ERK1)/ Extracellular-signal-regulated kinase 2 (ERK2), as part of the Ras-Raf-MEK-ERK signal transduction cascade, are involved in regulating a wide range of cellular processes, including cell adhesion, cell cycle progression, cell migration, cell survival, differentiation, metabolism, proliferation, and transcriptional control. Additionally, ERK1 signaling contributes to the regulation of cyclin D1 expression during the cell cycle. MAP kinases play a crucial role in inflammation and immune responses by regulating the production of pro- and anti-inflammatory cytokines, as well as prostaglandins, in response to lipopolysaccharide (LPS) stimulation.

General description

Research area: Cell Signaling

Recombinant, full-length, human MAP Kinase 1/Erk1 containing an N-terminal GST tag

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Packaging

Also available in 250μg size as catalog # 14-439M - Call for pricing and availability.

Physical form

Purified by glutathione-agarose. Activated with MEK1 and repurified on Ni2+/NTA agarose

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Signal transduction pathways involved in phosphorylation and activation of p70S6K following exposure to UVA irradiation.

Y Zhang et al.

The Journal of biological chemistry, 276(24), 20913-20923 (2001-03-30)

Ultraviolet light A (UVA) plays an important role in the etiology of human skin cancer, and UVA-induced signal transduction has a critical role in UVA-induced skin carcinogenesis. The upstream signaling pathways leading to p70(S6K) phosphorylation and activation are not well

Vav1 transduces T cell receptor signals to the activation of the Ras/ERK pathway via LAT, Sos, and RasGRP1.

Lucinda F Reynolds et al.

The Journal of biological chemistry, 279(18), 18239-18246 (2004-02-07)

Vav1 is a signaling protein required for both positive and negative selection of CD4(+)CD8(+) double positive thymocytes. Activation of the ERK MAPK pathway is also required for positive selection. Previous work has shown that Vav1 transduces T cell receptor (TCR)

Global Trade Item Number

SKU	GTIN
14-439M	04053252403972
14-439	04053252742989

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