14-444-M

PKC θ Protein, active, 10 µg

Name: PKC θ Protein, active, 10 µg
Brand: MM

Active, recombinant full-length human PKC theta with a C-terminal 6His-tag. For use in Kinase Assays.

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.41

eCl@ss:

32160405

Product Name

PKC θ Protein, active, 10 µg, Active, recombinant full-length human PKC theta with a C-terminal 6His-tag. For use in Kinase Assays.

biological source

human

recombinant

expressed in Sf21 cells

mol wt

M_w 83 kDa

manufacturer/tradename

Upstate^®

technique(s)

activity assay: suitable (kinase)

Analysis Note

routinely evaluated by phosphorylation of Histone H1 substrate in vitro

Biochem/physiol Actions

Protein Target: PKCθ

Target Sub-Family: AGC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Product Source: expressed in Sf21 cells

Recombinant full-length human PKC theta with a C-terminal 6His-tag

PKC Lipid activator (Catalog # 20-133) is required for use.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Physical form

immobilized nickel affinity chromatography

Preparation Note

6 months at -70 °C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Molecular cloning and characterization of PKC theta, a novel member of the protein kinase C (PKC) gene family expressed predominantly in hematopoietic cells.

G Baier et al.

The Journal of biological chemistry, 268(7), 4997-5004 (1993-03-05)

Members of the protein kinase C (PKC) family of serine/threonine kinases play a key role in regulating the differentiation and growth of diverse cell types and, to date, the cloning of seven mammalian PKC genes encoding eight distinct isoforms has

Complex formation and cooperation of protein kinase C theta and Akt1/protein kinase B alpha in the NF-kappa B transactivation cascade in Jurkat T cells.

B Bauer et al.

The Journal of biological chemistry, 276(34), 31627-31634 (2001-06-19)

Protein kinase C theta (PKC theta) is known to induce NF-kappa B, an essential transcriptional element in T cell receptor/CD28-mediated interleukin-2 production but also T cell survival. Here we provide evidence that PKC theta is physically and functionally coupled to

Global Trade Item Number

SKU	GTIN
14-444	04053252270130
14-444M	04053252516344

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