14-451-M

ROKα/ROCK-II Protein, active, 10 µg

Name: ROKα/ROCK-II Protein, active, 10 µg
Brand: MM

Active, N-terminal His6-tagged recombinant human ROCK-II residues 11-552, for use in Kinase Assays.

Synonym(s):

ROK, Renal carcinoma antigen NY-REN-35, Rho kinase, Rho-associated, coiled-coil-containing protein kinase 1, p160ROCK

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.41

eCl@ss:

32160405

Product Name

ROKα/ROCK-II Protein, active, 10 µg, Active, N-terminal His6-tagged recombinant human ROCK-II residues 11-552, for use in Kinase Assays.

biological source

human

recombinant

expressed in baculovirus infected Sf21 cells

form

liquid

shelf life

6 mo.

mol wt

M_w 63.3 kDa

manufacturer/tradename

Upstate^®

technique(s)

activity assay: suitable (kinase)

solubility

soluble

NCBI accession no.

NM_005406.2

UniProt accession no.

Q13464

storage temp.

−70°C

Quality Level

100

Gene Information

human ... ROCK1(6093)

Analysis Note

routinely evaluated by phosphorylation of the Long S6 Kinase substrate peptide

Biochem/physiol Actions

Protein Target: ROKα/ROCK-II
Target Sub-Family: AGC

ROCK (Rho kinase) is activated by interacting with Rho GTPase enzyme. ROCK plays a crucial role in various essential cellular functions like adhesion, contraction, migration, and proliferation. Moreover, it regulates the cytoskeleton and actomyosin contractility during apoptosis. Overexpression of ROCK protein has been observed in various types of cancer such as osteosarcoma, hepatocellular carcinomas, colon and bladder cancers.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Research area: APOPTOSIS

N-terminal His6-tagged recombinant human ROCK-II residues 11-552
Product Source: expressed by baculovirus in Sf21 insect cells

Rho kinase (ROCK) is a type of serine/threonine kinase. There are two isoforms, ROCK1 and ROCK2. In humans ROCK1 and ROCK2 are composed of 33 exons and located on chromosome 18 (18q11.1). The ROCK protein structure is characterized by a catalytic kinase domain and Rho-binding domain (RBD) at N-terminal and pleckstrin-homology (PH) domain and an internal cysteine-rich (CR) domain at C-terminal.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Packaging

Also available in 250μg size (14-451M), please inquire for pricing and availability.

Physical form

Ni²⁺/NTA-agarose

Preparation Note

6 months at -70°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Transformation mediated by RhoA requires activity of ROCK kinases.

E Sahai et al.

Current biology : CB, 9(3), 136-145 (1999-02-18)

The Ras-related GTPase RhoA controls signalling processes required for cytoskeletal reorganisation, transcriptional regulation, and transformation. The ability of RhoA mutants to transform cells correlates not with transcription but with their ability to bind ROCK-I, an effector kinase involved in cytoskeletal

Rho-associated kinase of chicken gizzard smooth muscle.

J Feng et al.

The Journal of biological chemistry, 274(6), 3744-3752 (1999-01-28)

Rho-associated kinase (Rho-kinase) from chicken gizzard smooth muscle was purified to apparent homogeneity (160 kDa on SDS-polyacrylamide gel electrophoresis) and identified as the ROKalpha isoform. Several substrates were phosphorylated. Rates with myosin phosphatase target subunit 1 (MYPT1), myosin, and the

Global Trade Item Number

SKU	GTIN
14-451M	04053252673061
14-451	04053252628160

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