14-602-M

PI3 Kinase (p110α/p85α) Protein, active, 20 µg

Name: PI3 Kinase (p110α/p85α) Protein, active, 20 µg
Brand: MM

Active complex of N-terminal His6-tagged recombinant full-length human p110α & untagged, recombinant, full length, human p85α. For use in Kinase Assays.

Synonym(s):

Active PI3 Kinase Protein, PI3 Kinase p110α/p85α

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.26

eCl@ss:

32160405

Product Name

PI3 Kinase (p110α/p85α) Protein, active, 20 µg, Active complex of N-terminal His6-tagged recombinant full-length human p110α & untagged, recombinant, full length, human p85α. For use in Kinase Assays.

biological source

human

recombinant

expressed in baculovirus infected Sf21 cells

mol wt

M_w 125.3 kDa (p110α)
M_w 83.7 kDa (p85α)

manufacturer/tradename

Upstate^®

technique(s)

activity assay: suitable (kinase)

Preparation Note

6 months at -70°C

Analysis Note

routinely evaluated by PIProfiler

Application

PI3 Kinase (p110α/p85α) Protein has been used in in vitro kinase assay.

Biochem/physiol Actions

Phosphoinositide 3-kinases (PI3Ks) are enzymes that play a crucial role in cell signaling. They are responsible for activating signaling pathways that regulate important cellular processes such as cell survival, proliferation, protein synthesis, cell metabolism, cytoskeletal rearrangements, and vesicle trafficking. PI3Ks exhibits both lipid kinase activity (phosphorylation of inositol ring in phosphatidylinositol (PtdIns) lipids) and protein kinase activity (including autophosphorylation).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Product Source: Coexpressed by baculovirus in Sf21 insect cells

Research area: Cell signaling

Protein Target: PI3Kα/p85α Target Sub-Family: PI3K/PI4K

Complex of N-terminal His6-tagged recombinant full-length human p110α and untagged, recombinant, full length, human p85α

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Packaging

Also available in 250μg size as catalog # 14-602M - Call for pricing and availability.

Physical form

Ni²⁺/NTA-agarose

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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PKCalpha reduces the lipid kinase activity of the p110alpha/p85alpha PI3K through the phosphorylation of the catalytic subunit

Sipeki S, et al.

Biochemical and Biophysical Research Communications, 339(1), 122-125 (2006)

Kinetic and structural analyses reveal residues in phosphoinositide 3-kinase ? that are critical for catalysis and substrate recognition

Maheshwari S, et al.

The Journal of biological chemistry, 292(33), 13541-13550 (2017)

Oncogenic mutations of p110α isoform of PI 3-kinase upregulate its protein kinase activity.

Christina M Buchanan et al.

PloS one, 8(8), e71337-e71337 (2013-08-13)

In addition to lipid kinase activity, the class-I PI 3-kinases also function as protein kinases targeting regulatory autophosphorylation sites and exogenous substrates. The latter include a recently identified regulatory phosphorylation of the GM-CSF/IL-3 βc receptor contributing to survival of acute

Global Trade Item Number

SKU	GTIN
14-602	04053252405501
14-602M	04053252372551

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