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Merck

1.46476

ReadyTube® Half Fraser Broth

ReadyTube®, bottle capacity 250 mL, bottle filling volume 225 mL, closure type, blue screw cap with 3 loci, box of 6 bottles, selective for Listeria monocytogenes

Synonym(s):

Fraser Broth

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About This Item

NACRES:
NA.74
UNSPSC Code:
41106212
Bottle capacity:
250 mL
Bottle filling volume:
225 mL
Feature:
closure type blue screw cap with 3 loci
ready-to-use
Agency:
ISO 11190
Ph:
7.2 ( in H2O)

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Product Name

ReadyTube® Half Fraser Broth, ReadyTube®, bottle capacity 250 mL, bottle filling volume 225 mL, closure type, blue screw cap with 3 loci, box of 6 bottles, selective for Listeria monocytogenes

agency

ISO 11190

form

liquid (ready-to-use)

feature

closure type blue screw cap with 3 loci
ready-to-use

packaging

box of 6 bottles

manufacturer/tradename

ReadyTube®

bottle capacity

250 mL

bottle filling volume

225 mL

pH

7.2 ( in H2O)

Quality Level

300

application(s)

environmental
food and beverages
microbiology

storage temp.

2-8°C

suitability

selective for Listeria monocytogenes

Related Categories

Analysis Note

This medium contains peptone, casein, yeast extract, and meat extract, which provide essential nutrients like carbon and nitrogenous compounds, including vitamins, amino acids, and trace ingredients. Phosphates buffer the medium while sodium chloride maintains osmotic equilibrium. Nalidixic acid and acriflavine inhibit the growth of Gram-negative and Gram-positive organisms respectively, except Listeria sp. Listeria sp. hydrolyze esculin to glucose and esculetin. The latter combines with ferric ions of ferric ammonium citrate, resulting in the formation of 6-7-Dihydroxycoumarin, a black-brown complex. Ferric ammonium citrate also enhances the growth of L. monocytogenes. The high salt tolerance (of sodium chloride) of Listeria is used as means to inhibit the growth of Enterococci. Lithium chloride is also used to inhibit Enterococci, which also possess the ability to hydrolyze esculin.

Application

ReadyTube® Fraser broth is used for the primary and secondary selective enrichment of Listeria sp. from food and animal feed as well as from environmental samples and other materials.

General description

ReadyTube® is ready to use media to provide the highest level of quality and testing confidence for improving accuracy and significantly reducing the risk of false results. ReadyTube® Half Fraser broth is a selective enrichment medium designed to help recovery of Listeria sp.

Legal Information

READYTUBE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Protocols

Listeria: Surviving Bacteria

Listeria is a public health concern, affecting elderly, pregnant, newborns, and immunocompromised individuals.

Standards for Detection and Enumeration of Listeria monocytogenes and Listeria spp. in the food chain

Discover the revised EN ISO 11290-1 and EN ISO 11290-2 standards and gain insights into updated detection methods for Listeria monocytogenes and Listeria spp., including enumeration in food, animal feed, and environmental samples. Explore culture media solutions.

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