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Key Documents

14-814

Sigma-Aldrich

UbcH8 Conjugating Enzyme, 100 µg

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About This Item

UNSPSC Code:
12352204
eCl@ss:
32160405
NACRES:
NA.32

biological source

human

Quality Level

mol wt

Mw 18 kDa

manufacturer/tradename

Upstate®

NCBI accession no.

UniProt accession no.

General description

UbcH8 is a class I ubiquitin-conjugating (E2) enzyme highly similar in structure to UbcH7. UbcH8 is a primary target gene of interferon-alpha and gamma, and recent studies have indicated that UbcH8 is the E2 enzyme for the ubiquitin-like protein ISG15. These studies suggest overlap between the ISG15 and ubiquitin conjugation pathways, and have important implications for understanding the regulation and function of ISG15 in the IFN alpha/beta response. Typical enzyme concentration to support conjugation in vitro is 100nM to 1μM depending upon conditions.
expressed in E. coli

Application

UbcH8 is a class I ubiquitin-conjugating (E2) enzyme highly similar in structure to UbcH7. UbcH8 is a primary target gene of interferon-alpha & gamma.

Physical form

Format: Purified using Ni2+ chelation chromatography with Ni2+ in limiting concentration.

Preparation Note

For long term storage store at -80°C.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1


Certificates of Analysis (COA)

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