14-856
Ubiquitin C-terminal hydrolase L3 (UCH-L3), 50 µg
From human cDNA, expressed in E. coli. Ubiquitin C-terminal hydrolases are a family of cysteine hydrolases that catalyze the hydrolysis of amides, esters & thioesters of the C-terminus of ubiquitin.
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
eCl@ss:
32160405
Specific activity:
>1000 pmol/min-μg, 25 °C (with 1 μM ubiquitin-AMC as substrate and UCH-L3 at 20 pM (0.58 ng/mL).)
Biological source:
human
Recombinant:
expressed in E. coli
biological source
human
Quality Segment
recombinant
expressed in E. coli
specific activity
>1000 pmol/min-μg, 25 °C (with 1 μM ubiquitin-AMC as substrate and UCH-L3 at 20 pM (0.58 ng/mL).)
mol wt
Mw 26.2 kDa
manufacturer/tradename
Upstate®
NCBI accession no.
UniProt accession no.
shipped in
dry ice
General description
Produced from human cDNA, expressed in E. coli. Ubiquitin C-terminal hydrolases (UCHs) are a family of cysteine hydrolases that catalyze the hydrolysis of amides, esters and thioesters of the C-terminus of ubiquitin. UCH-L3 is a member of the lower molecular weight group of UCHs involved in the hydrolysis of small C-terminal derivatives of ubiquitin that form non-specifically during the process of protein ubiquitinylation.
Product Source: Recombinant human UCH-L3 expressed in E.coli.
Application
From human cDNA, expressed in E. coli. Ubiquitin C-terminal hydrolases are a family of cysteine hydrolases that catalyze the hydrolysis of amides, esters & thioesters of the C-terminus of ubiquitin.
Preparation Note
Store at -70°C for up to 12 months from date of receipt. As supplied, the enzyme is stable on ice for several hours. Activity is stable up to 6 freeze/thaw cycles (snap freezing in a dry/ice ethanol bath or liquid nitrogen).
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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