14-921
CpG MethylQuest®Protein (Recombinant GST-MBD fusion protein), 100 µg
The CpG MethylQuest protein is an effective & versatile tool for the study of CpG methylation in DNA. This purified recombinant protein contains the methyl binding domain of the mouse MBD2b protein fused to a GST from S japonicum.
Synonym(s):
Methyl Binding domain protein 2b, MBD2b, Methyl CpG binding domain protein
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About This Item
Product Name
CpG MethylQuest®Protein (Recombinant GST-MBD fusion protein), 100 µg, The CpG MethylQuest protein is an effective & versatile tool for the study of CpG methylation in DNA. This purified recombinant protein contains the methyl binding domain of the mouse MBD2b protein fused to a GST from S japonicum.
recombinant
expressed in E. coli
assay
~95% (SDS-PAGE)
manufacturer/tradename
CpG MethylQuest®
concentration
1 mg/mL
technique(s)
DNA purification: suitable
shipped in
dry ice
Analysis Note
General description
MBD2b protein fused to a glutathione-S-transferase protein (GST) from S japonicum. These two protein domains are separated by a linker containing a thrombin cleavage site.
This unbound protein has several unique properties that can be incorporated into your own customized assays for the study of DNA methylation.
- Competitive elution of the GST-MBD protein or GST-MBD-methylated DNA complexes with glutathione.
- Elution of intact complexes by thrombin digestion
- Immobilization of protein DNA complexes or GST-MBD protein to other solid surfaces such as microtiter plates coated with GST antibodies.
- Visualization of complexes using labeled GST antibodies or GST antibodies and labeled secondary antibodies.
- Surface cysteine groups on the GST protein can be chemically modified to allow the addition of other reporters, affinity tags, or functional groups.
To simplify the isolation of methylated DNA, this protein can also be obtained pre-bound to magnetic beadsas a component of the CpG MethylQuest DNA Isolation Kit (17-10035).
Physical form
Preparation Note
Legal Information
Storage Class
10 - Combustible liquids
wgk
WGK 1
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Related Content
DNA methylation is an important epigenetic mechanism regulating gene silencing, imprinting, embryonic development, and chromosome stability. DNA methylation occurs on the 5 carbon position of cytosine residues mainly within CpG dinucleotides to form 5-methylcytosines (5-mC). The reaction is catalyzed by DNA methyltransferases (DNMTs). 5-methylcytosines residues may also be hydroxylated by TET enzymes to form 5-hydroxymethylcytosine (5-hmC), which has differing roles from 5-mC. EMD Millipore provides robust tools that enable you to not only detect and quantify 5-mC and 5-hmC, but also to accurately distinguish between these modifications.
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