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1.50357

Purospher®STAR Si (5 µm) HPLC Columns

L × I.D. 250 mm x 4.6 mm LiChroCART™ HPLC cartridge, manuCART™ holder required for use

Synonym(s):

Purospher® STAR SI (5 µm) LiChroCART™ 250-4.6

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52
Particle size:
5 μm
Matrix active group:
Silica bonding phase
Pore size:
120 Å pore size
Matrix:
fully porous particle
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Product Name

Purospher® STAR Si (5 µm), L × I.D. 250 mm x 4.6 mm LiChroCART™ HPLC cartridge, manuCART™ holder required for use

material

stainless steel column

Quality Level

100

agency

suitable for USP L3

product line

Purospher®

feature

endcapped: no

packaging

1 ea of

parameter

250 bar pressure, 65 °C max. temp.

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

column L × I.D.

25 cm × 4.6 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

Silica bonding phase

particle size

5 μm

pore size

120 Å pore size

operating pH range

2-7.5

separation technique

hydrophilic interaction (HILIC), normal phase

storage temp.

no temp limit

General description

Purospher® STAR Si endcapped, 5 µm HPLC columns offers highest separation efficiency for normal-phase chromatography of low molecular weight compounds soluble in organic solvents.Purospher® STAR Si, 5 µm LiChroCART™ 250-4.6 is a cartridge column which requires a manuCART™ cartridge column holder part number 1.51486.0001. The manuCART™ can be used to hold one cartridge column with or without direct integration of a 4-4 mm guard column. The recommended guard column is Purospher® STAR RP-8 endcapped (5 µm) LiChroCART™ 4-4 part number 1.50249.0001. LiChroCART™ cartridge columns mounted with a manuCART™ cartridge column holder is hand-tight only and have therefore a maximum pressure stability of 250 bar. For higher pressure stability requirements Purospher® STAR Si, 5 µm Hibar® RT 250-4.6 HPLC columns are available part number 1. 51911.0001.The column dimension of 250 mm length x 4.6 mm I.D. is the optimal choice for the HPLC separation of complex samples allowing significant solvent savings and improved sensitivity.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used for the separation of aflatoxins, carotenoids, oils, fat-soluble vitamins, lipids, and fatty acids.

Features and Benefits

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Use with Normal-phase or HILIC conditions (needs to be flushed to HILIC mobile phase)
  • Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 60 bar
Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 60000
Symmetry (2-Nitrotoluene): 0.8 - 1.4
Capacity factor (2-Nitrotoluene): 0.5 - 2.0

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

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