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1.50438

SeQuant® ZIC®-pHILIC (5 µm) HPLC Columns

L x I.D. 20 mm x 2.1 mm guard Column Kit (3 pc) with coupler (1 pc)

Synonym(s):

Hydrophilic Interaction Liquid Chromatography Guard Column

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52
Particle size:
5 μm
Matrix active group:
ZIC-HILIC, Zwitterion bonding phase, ZIC-pHILIC
Matrix:
polymeric particle platform
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Product Name

SeQuant® ZIC®-pHILIC Guard Kit 20 x 2.1 mm, PEEK-lined stainless steel column, pkg of 3 column

material

PEEK-lined stainless steel column

Quality Segment

agency

suitable for USP L122

product line

SeQuant®

feature

endcapped: no

analyte functional class(es)

antibiotics, sweeteners, water soluble vitamins

analyte chemical class(es)

PFAS, alcohols / fermentation, aliphatic amines, alkaloids, amino acids, aromatic amines, carboxylic acids as methyl esters, esters, glycans, glycols, inorganic ions, monosaccharides, nitrosamines, nucleosides, oligonucleotides, organic phosphates, peptides, pesticides, phospholipids, polysaccharides, small carbohydrates, sugar alcohols, sulfonamides

packaging

pkg of 3 column

parameter

200 bar pressure, 50 °C max. temp.

technique(s)

LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)

column L × I.D.

2 cm × 2.1 mm

matrix

polymeric particle platform

matrix active group

ZIC-HILIC, Zwitterion bonding phase, ZIC-pHILIC

particle size

5 μm

operating pH

2-10

separation technique

hydrophilic interaction (HILIC)

storage temp.

no temp limit

General description

The Guard column kit contains 3 SeQuant® ZIC®-pHILIC guard columns including the fitting to connect to the analytical column.SeQuant® ZIC®-pHILIC stationary phase has the same highly polar, bonded, permanent zwitterion, functional group as the silica based ZIC®-HILIC Column. The user can therefore expect the same selectivity, however, with a trade-off in flow-rate range, and separation efficiency, common with polymeric materials. The more durable support allows for use in an extended pH range, which can be beneficial for certain applications.SeQuant® ZIC®-pHILIC HPLC columns are the preferred choice when extreme eluent conditions are needed to overcome challenging separations of polar hydrophilic compounds. Offering excellent pH stability from pH 2 to 10, these polymer-based columns carry densely bonded, truly zwitterionic functional groups with a charge balance of 1:1. This results in straightforward and reproducible separations with wide-spectrum pH tuning for optimal separation selectivity and detection sensitivity. Furthermore, SeQuant® ZIC®-pHILIC columns minimize background noise in evaporative detection techniques with high sensitivity to ions, such as charged aerosol detectors (CAD). SeQuant® ZIC®-pHILIC columns are available with 5 μm polymer particles in various formats of analytical dimensions. Alternative choices for the separation of polar hydrophilic compounds include silica-based SeQuant® ZIC®-HILIC columns with identical functional groups, and SeQuant® ZIC®-cHILIC columns with complementary selectivity.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Analysis Note

performance test cytosine
Extent of testing: conforms

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

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