1.50490
SeQuant® ZIC®-HILIC 200Å (5 µm) HPLC Columns
L × I.D. 5 mm × 1.0 mm guard Columns (5 pc)
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About This Item
UNSPSC Code:
41115709
NACRES:
NB.21
Particle size:
5 μm
Matrix active group:
ZIC-HILIC bonding phase
Pore size:
100 Å pore size
Matrix:
fully porous particle
Product Name
SeQuant® ZIC-HILIC™ (5µm) 5 x 1 mm (5 pc), column L × I.D. 0.5 cm × 1 mm
material
stainless steel column
agency
suitable for USP L114
product line
SeQuant®
feature
endcapped: no
parameter
400 bar pressure, 70 °C max. temp.
technique(s)
HPLC: suitable, LC/MS: suitable (Capillary LC), mass spectrometry (MS): suitable (High)
column L × I.D.
0.5 cm × 1 mm
matrix
fully porous particle
matrix active group
ZIC-HILIC bonding phase
particle size
5 μm
pore size
100 Å pore size
operating pH
2-7.5
separation technique
hydrophilic interaction (HILIC)
storage temp.
no temp limit
General description
The SeQuant® ZIC®-HILIC Guard column protects your Capillary column from contamination. The pack contains 5 Guard columns.SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide
Legal Information
SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany
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Certificates of Analysis (COA)
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